Maternal miR-202-5p is required for zebrafish primordial germ cell migration by protecting small GTPase Cdc42  

作　　者：Yilin Jin Wei Liu Yangxi Xiang Wanwan Zhang Hong Zhang Kuntong Jia Meisheng Yi 

机构地区：[1]School of Marine Sciences Sun Yat-sen University,Guangzhou 519082,China [2]Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering,Sun Yat-sen University,Guangzhou 519082,China [3]Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai),Sun Yat-sen University,Guangzhou 519082,China

出　　处：《Journal of Molecular Cell Biology》2020年第7期530-542,共13页分子细胞生物学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(31771587 and 31970535);Fundamental Research Funds for the Central Universities(17lgpy66);the Pearl River S&T Nova Program of Guangzhou(201806010047);the Scince and Technology Planning Project of Guangdong Province(2017A030303010);the Guangdong Natural Science Foundation(2015A030308012).

摘　　要：In many lower animals,germ cell formation,migration,and maintenance depend on maternally provided determinants in germ plasm.In zebrafish,these processes have been extensively studied in terms of RNA-binding proteins and other coding genes.The role of small non-coding RNAs in the regulation of primordial germ cell(PGC)development remains largely unknown and poorly investigated,even though growing interests for the importance of miRNAs involved in a wide variety of biological processes.Here,we reported the role and mechanism of the germ plasm-specific miRNA miR-202-5p in PGC migration:(i)both maternal loss and knockdown of miR-202-5p impaired PGC migration indicated by the mislocalization and reduced number of PGCs;(ii)cdc42se1 was a direct target gene of miR-202-5p,and overexpression of Cdc42se1 in PGCs caused PGC migration defects similar to those observed in loss of miR-202-5p mutants;(iii)Cdc42se1 not only interacted with Cdc42 but also inhibited cdc42 transcription,and overexpression of Cdc42 could rescue PGC migration defects in Cdc42se1 overexpressed embryos.Thus,miR-202-5p regulates PGC migration by directly targeting and repressing Cdc42se1 to protect the expression of Cdc42,which interacts with actin to direct PGC migration.

关 键 词：primordial germ cell miR-202-5p Cdc42se1 CDC42 MIGRATION 

分 类 号：Q34[生物学—遗传学] Q492