辣椒CaWRKY14转录因子的生物学特性研究  

Biological Characteristics of CaWRKY14 Transcription Factor in Pepper

作　　者：王倩倩 刁卫平[2] 潘宝贵[2] 郭广君[2] 刘金兵[2] 王述彬[1,2] WANG Qianqian;DIAO Weiping;PAN Baogui;GUO Guangjun;LIU Jinbing;WANG Shubin(College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China;Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014, China)

机构地区：[1]南京农业大学园艺学院,南京210095 [2]江苏省农业科学院蔬菜研究所,江苏省高效园艺作物遗传改良重点实验室,南京210014

出　　处：《西北植物学报》2020年第9期1498-1504,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家重点研发计划(2017YFD0101901);国家自然科学基金(31601768);江苏省农业重大新品种创制项目(PZCZ201714);现代农业产业技术体系建设专项资金(CARS-23-G42)。

摘　　要：WRKY转录因子是植物响应病原菌胁迫最重要的转录因子之一,且参与抗病反应及信号传导通路的调控。为研究辣椒WRKY基因的生物学特征,以辣椒高抗疫病材料CM334为试材,克隆获得响应疫霉菌诱导的转录因子CaWRKY14。生物信息学分析表明,该基因DNA全长2530 bp,cDNA全长1662 bp,含有5个内含子,编码553个氨基酸,含有1个WRKY保守结构域,属于GroupⅡ(b)。实时荧光定量表达分析表明,CaWRKY14不仅受ABA和疫霉菌胁迫诱导表达,且表达量分别在12 h和24 h时达到峰值,分别是对照的8.54和8.04倍,同时也受高盐、热激和干旱胁迫诱导。利用VIGS技术对CaWRKY14转录因子进行沉默后发现,抗病材料CM334接种疫霉菌后趋于发病。研究表明,CaWRKY14基因在辣椒响应疫霉菌胁迫进程中可能发挥着重要作用。WRKY transcription factor is one of the most important transcription factors for plants in response to pathogenic bacteria stress,and participates in disease resistance and the regulation of signal transduction pathways.In order to study the biological characteristics of pepper WRKY genes,we used the pepper highly resistant disease material CM334 as the test material to clone and obtain the transcription factor CaWRKY14 in response to Phytophthora infestans.Bioinformatic analysis showed that the gene has a total DNA length of 2530 bp and a cDNA length of 1662 bp.It contains 5 introns,encodes 553 amino acids,and contains a WRKY conserved domain.Real-time fluorescence quantitative expression analysis showed that CaWRKY14 was not only induced by ABA and Phytophthora capsici stress,the expression level reached the peak at 12 h and 24 h,respectively,8.54 and 8.04 times of the control.It is also induced by high salt,heat shock and drought stress.In addition,after silencing CaWRKY14 transcription factor using VIGS technology,it was found that the disease-resistant material CM334 tended to develop disease after inoculation with Phytophthora infestans.Studies have shown that CaWRKY14 gene may play an important role in the process of pepper response to Phytophthora capsici.

关 键 词：辣椒 CaWRKY14 克隆 胁迫 生物学特性 

分 类 号：Q785[生物学—分子生物学] Q786