牛大力HPLC指纹图谱的研究  

Study on HPLC Fingerprint of Millettia speciosa Champ

作　　者：苏志恒[1] 郑华[3] 宋慧[1] 王捷 梁永红[1] 阮俊翔 Su Zhiheng;Zheng Hua;Song Hui;Wang Jie;Liang Yonghong;Ruan Junxiang(Harmaceutical College,Guangxi Medical University,Nanning 530021,China;Guangxi Yuhualing Technology Development Co.,Ltd.,Nanning 530000,China;Life Sciences Institute of Guangxi Medical University,Nanning 530021,China;Harmaceutical Factory of Guangxi Medical University,Nanning 530021,China)

机构地区：[1]广西医科大学药学院,广西南宁530021 [2]广西宇花灵科技发展有限公司,广西南宁530000 [3]广西医科大学医学科学实验中心,广西南宁530021 [4]广西医科大学制药厂,广西南宁530021

出　　处：《现代食品》2020年第17期205-209,共5页Modern Food

基　　金：基于微观组效关系的牛大力抗抑郁药效物质基础及作用机制研究(编号:2018GXNSFAA138096);南宁市青秀区科学研究与技术开发计划项目重大专项“开放式广西特色中药民族药研发平台建设的研究”(编号:2015Z01)。

摘　　要：目的:建立牛大力的HPLC指纹图谱鉴别方法,为其质量控制提供参考。方法:Agilent Eclipse XDB-C18(5μm,4.6 mm×250 mm)色谱柱为固定相,以0.05%磷酸水溶液-乙腈为流动相进行梯度洗脱,检测波长为220 nm,柱温为30℃,流速为1.0 mL·min-1,进样体积为5μL,采用中药色谱指纹图谱相似度评价系统(2004A)对13批牛大力指纹图谱进行分析。结果:以高丽槐素为参照峰,初步建立牛大力HPLC指纹图谱,共有15个共有峰,13批牛大力药材的HPLC指纹图谱相似度在0.782~0.994。结论:该方法操作简便,精密度、重复性和稳定性良好,可作为牛大力质量控制的有效方法。Objective:To establish an HPLC fingerprint method of Milletita speciosa Champ.,and provide reference for quality control of it.Methods:HPLC fingerprint were determined on Agilent Eclipse XDB-C18(5μm,4.6 mm×250 mm)column with mobile phase consisting of acetonitrile-0.05%phosphoric acid water with gradient elution;flow rate was 1.0 mL·min-1;column temperature was 30℃and detection wavelength was set at 220 nm,injection volume was 5μL.The similarity of chemical fingerprinting of Millettia speciosa Champ.from 13 different place of origin were compared by using“Traditional Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System”(2004 A).Results:The HPLC fingerprint was established with 15 common peaks,the similarity of the HPLC fingerprint of 13 batches of Millettia speciosa Champ was 0.782~0.994.Conclusion:The method is reproducible,precise and steady,which can be used for quality control of Milletia speciosa Champ.

关 键 词：牛大力 HPLC 指纹图谱 相似度评价 质量控制 

分 类 号：R853.75[医药卫生—航空、航天与航海医学]