PM2.5通过ROS-Nrf2/NF-κB信号通路诱导人支气管上皮细胞炎症反应  

PM induces inflammation in human bronchial epithelial cells through ROS-Nrf2 signaling pathway

作　　者：邹杨 李淑珍 李新鸣[1] 杨彪[1] 孙冶[1] 肖纯凌[1] ZOU Yang;LI Shu-zhen;LI Xin-ming(Key Laboratory of Environmental Pollution and Health,Shenyang Medical College,Shenyang,Liaoning Province 110034,China;不详)

机构地区：[1]沈阳医学院病原生物学教研室,辽宁沈阳110034 [2]沈阳医学院医学免疫学教研室

出　　处：《中国公共卫生》2020年第10期1438-1442,共5页Chinese Journal of Public Health

基　　金：沈阳医学院研究生创新基金(Y20180501);辽宁省科学技术厅项目(2017225076);沈阳市科技局“重大科技研发项目”(18-400409)。

摘　　要：目的探讨PM2.5暴露对人支气管上皮细胞(16-HBE)炎症反应的影响。方法2018年12月选择辽宁省沈阳市黄河北大街为采样地点收集空气中的细颗粒物,用浓度为12.5、25、50和100μg/mL PM2.5分别处理16-HBE细胞0、6、12和24 h,采用CCK-8检测16-HBE细胞活性。酶联免疫吸附法(ELISA)检测细胞上清液中白介素–1(IL-1)、白介素–6(IL-6)、肿瘤坏死因子–α(TNF-α)、基质金属蛋白酶–9(MMP-9)的变化水平,荧光探针法检测细胞活性氧(ROS)相对水平,免疫印迹(WB)法检测细胞蛋白核因子E2相关因子2(Nrf2)和核因子–KB(NF-κB)通路中的IKK和P65的水平。结果PM2.5能够诱导人支气管上皮细胞增殖活性的降低和炎症反应的发生,且具有时间依赖性和剂量依赖性。结论PM2.5暴露通过ROS-Nrf2/Nf-kb信号通路诱导人呼吸道上皮细胞数量的减少和炎症反应的发生。Objective to investigate the effect of particulate matter less than 2.5μm in aerodynamic diameter(PM2.5)on inflammatory response of human bronchial epithelial cells(16-HBE).Methods PM2.5 samples in ambient air was collected in Shenyang city of Liaoning province in December 2018.The 16-HBE cells were treated with sampled PM2.5 at concentrations of 12.5,25,50 and 100 g/mL for 0,6,12,and 24 hours,respectively.Cell counting kit-8(CCK-8)assay was used to detect the activity of the 16-HBE cells.The contents of interleukin-1β(IL-1β),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),and matrix metalloproteinase-9(MMP-9)in cell culture supernatant were detected with enzyme-linked immunosorbent assay(ELISA).The relative levels of cell reactive oxygen species(ROS)were detected with fluorescence probe and the levels of I kappa B kinase(IKK)and P65 in the cell protein nuclear factor erythroid-2 related factor 2(Nrf2)and nuclear factor-kappa B(NF-κB)pathway were detected with Western blot.Results PM2.5 can induce a decrease in proliferation and occurrence of inflammatory reaction of 16-HBE cells in time-and dose-dependent manner.Conclusion PM2.5 exposure can inhibit the proliferation and induce inflammatory reaction of human respiratory epithelial cells through ROS-Nrf2/Nf-kb signaling pathway.

关 键 词：PM2.5 16-HBE 活性氧 Nrf2 NF-ΚB 炎性因子 

分 类 号：R122.7[医药卫生—环境卫生学]