地榆膏对大鼠深Ⅱ度烫伤的治疗作用  

Effect of Sanguisorba Plaster on DeepⅡDegree Burn in Rats

作　　者：桑龙燕 李莉[1] 程孝平[2] 谷梅 李响 叶伊琳[1] SANG Longyan;LI Li;CHENG Xiaoping;GU Mei;LI Xiang;YE Yilin(Anhui University of Chinese Medicine,Hefei 230012,China;Outpatient Department of Wound Stomatology,the First Affiliated Hospital of Anhui University of Chinese Medicine,Hefei 230012,China)

机构地区：[1]安徽中医药大学,安徽合肥230012 [2]安徽中医药大学第一附属医院伤口造口门诊,安徽合肥230031

出　　处：《云南中医学院学报》2020年第2期1-6,共6页Journal of Yunnan University of Traditional Chinese Medicine

基　　金：安徽中医药大学校级科研项目(RD17200079)。

摘　　要：目的观察地榆膏对深Ⅱ度烫伤大鼠模型创面愈合的影响,并探讨其作用机制。方法选取SD大鼠,随机分为正常组、模型组、美宝湿润烧伤膏组、莫匹罗星组、地榆膏组。除正常组外其余大鼠制备深Ⅱ度烫伤模型,造模成功后观察各组大鼠创面基本情况、创面愈合率、组织病理学变化、CD31蛋白相对表达量以及血清中IL-6和TNF-α水平。结果烫伤后7、14、21 d,地榆膏组大鼠创面基本情况和组织病理学变化情况良好,美宝湿润烫伤膏组次之,莫匹罗星组和模型组欠佳。与正常组比较,模型组血清中IL-6、TNF-α及组织中CD31的表达明显升高(P<0.05);而各药物处理组创面愈合率及CD31相对表达量明显高于模型组(P<0.05),血清中IL-6、TNF-α水平明显低于模型组(P<0.05)。烫伤后14、21 d,地榆膏组创面愈合率和CD31表达量明显高于莫匹罗星组(P<0.05),但与美宝湿润烫伤膏组比较差异无统计学意义。结论地榆膏能抑制炎性因子IL-6和TNF-α释放、促进创面微血管生成,进而起到促进烫伤创面愈合的作用。Objective To observe the effect of Sanguisorba Plaster on wound healing in a rat model of deep Ⅱ degree burn and explore its action mechanism. Methods SD Rats were randomly divided into normal group, model group,Mei-bao’s scald-plaster group, Mupirocin Ointment group and Sanguisorba Plaster group. All rats except the normal group were made deep Ⅱ degree scald model, and the wound healing rate, the change of histopathology, the relative expression of CD31 protein and the levels of IL-6 and TNF-α in serum were detected. Results On 7, 14, 21 d after burn, the wound surface and histopathology of rats in Sanguisorba Plaster group were good, followed by Mei-bao ’s scald-plaster group,Mupirocin Ointment group and model group. Compared with normal group, the levels of IL-6 and TNF-α in serum and CD31 in tissues were increased(P <0.05). And the wound healing rate and relative expressions of CD31 in drug treatment groups were significantly higher than those in model group(P <0.05), and the levels of IL-6 and TNF-α in serum were significantly lower than model group(P<0.05). On 14 d and 21 d after burn, the wound healing rate and relative expression of CD31 were significantly higher in Sanguisorba Plaster group than in Mupirocin Ointment group( P<0.05), while there was no difference when compared with Mei-bao’s scald-plaster group. Conclusion Sanguisorba Plaster could inhibit the release of inflammatory cytokines IL-6 and TNF-α, and enhance wound microangiogenesis, promote wound healing.

关 键 词：地榆膏 深Ⅱ度烫伤 白细胞介素-6 肿瘤坏死因子 CD31 

分 类 号：R285.5[医药卫生—中药学]