用高热量饲料建立大鼠肥胖模型的实验研究  

Experimental Study on Establishment of Obesity Model in Rats Induced by High-Calorie Diet

作　　者：吕晓君 吴森 张菊 徐小玲 潘望平 李厚钢 王平慧 何开勇 LV Xiaojun;WU Sen;ZHANG Ju;XU Xiaoling;PAN Wangping;LI Hougang;WANG Pinghui;HE Kaiyong(Hubei Institute for Drug Control,Wuhan 430064,China;Hubei Engineering Research Center for Drug Quality Control,Wuhan 430064,China)

机构地区：[1]湖北省药品监督检验研究院,武汉430064 [2]湖北省药品质量检测与控制工程技术研究中心,武汉430064

出　　处：《实验动物与比较医学》2020年第5期374-383,共10页Laboratory Animal and Comparative Medicine

基　　金：国家食品药品监督管理总局保健食品功能目录研究专项(ZBW-2017-BJSP-05)。

摘　　要：目的观察用高热量饲料建立大鼠肥胖模型的效果,以及两个品种大鼠的建模差异,并比较不同的肥胖评价方法和肥胖敏感动物筛选方法,为研究减肥产品的功能时制备动物模型提供参考。方法选用SD及Wistar雄性大鼠各120只,分别按体质量随机分为2组(空白对照组和模型组),每组60只。空白对照组给予维持饲料,模型组给予改进配方的高热量饲料,每周记录大鼠体质量和体长1次,每周称饲料质量2次,计算Lee’s指数、摄食量及食物利用率,共6周。喂养2周或6周后,每组分别选取30只大鼠检测血生化指标,解剖后取双侧肾周围脂肪和睾丸周围脂肪并称质量,计算脂/体比。分析大鼠体质量增加、Lee’s指数与肥胖和肥胖敏感的相关性,比较喂养2周时剔除体质量增加分别排在后1/3、10%、20%的大鼠后体质量增加与肥胖的相关性。结果与空白对照组比较,两种品系的模型组大鼠体质量、Lee’s指数、体质量增加、食物利用率、体内脂肪质量及脂/体比均增加,摄食量降低,血清葡萄糖和三酰甘油水平升高,高密度脂蛋白胆固醇水平降低(均P<0.05)。SD大鼠和Wistar大鼠比较,模型组大鼠的各指标无明显差异(P>0.05),空白对照组大鼠的体长、Lee’s指数和食物利用率偶而有差异(均P<0.05),空白对照组大鼠的血清高密度脂蛋白胆固醇水平在喂食2周和6周时有明显差异(P<0.05)。两种品种的大鼠体质量增加与肥胖及肥胖敏感的相关性均明显大于Lee’s指数与肥胖及肥胖敏感的相关性(均P<0.05);剔除体质量增加排在后10%和20%的大鼠时体质量增加与肥胖的相关性均明显好于剔除体质量增加排在后1/3的大鼠,其中剔除10%的相关系数最高。结论用改进的高热量饲料可成功建立SD和Wistar大鼠肥胖模型,且两个品种间无明显差异;体质量增加作为肥胖评价和肥胖敏感动物筛选的指标优于Lee’s指数,而且筛选肥胖敏感Objective To observe the effect of high-calorie diet on the establishment of obesity model in rats,and the differences in modeling between two different stocks of rats,and to compare the differences of obesity evaluation methods and obesity-sensitive animals screening methods,providing a reference for the preparation of animal models to study the function of weight loss products.Methods Total of 120 SD(Sprague Dawley)and 120 Wistar male rats were randomly divided into 2 groups(blank control group and model group)by body weight,with 60 rats in each group.The rats in blank control group were given basic diet,and the rats in model group were given improved high-calorie diet.The body weight and body length of the rats were recorded once a week,and the feeds were weighed twice a week to calculate the Lee’s index,food intake and food utilization for 6 weeks.After 2 weeks and 6 weeks of feeding,30 rats in each group were respectively selected to measure the biochemical indicators,and the fat around the kidney and the testis was dissected and weighed to calculate the fat/body weight ratio.The correlations of weight gain and Lee’s index with obesity and obesity sensitivity were analyzed.The correlation between obesity and weight gain after eliminating the last 1/3,10%,and 20% rats with weight gain after 2 weeks of feeding were compared.Results Compared with blank control group,the body weight,Lee’s index,weight gain,food utilization rate,body fat weight and fat/body weight ratio,as well as the serum levels of glucose and triglyceride in the two stocks of rats in model group were increased,while the food intake and the serum level of high density liptein cholesterol(HDL-C)were decreased(all P<0.05).There was no significant difference in each index of model group between SD rats and Wistar rats(P>0.05),but the differences in body length,Lee’s index and food utilization rate of the two stocks of rats in blank control group were occasionally appeared during the experiment(all P<0.05).There was significant difference i

关 键 词：高热量饲料 肥胖模型 SD大鼠 WISTAR大鼠 

分 类 号：Q95-33[生物学—动物学]