老年SD大鼠自发性肿瘤的病理学观察  

Pathological Observation of Spontaneous Tumor in Aged SD Rats

作　　者：周晓丽[1] 张倩[1] 高峥 钱智勇[1] ZHOU Xiaoli;ZHANG Qian;GAO Zheng;QIAN Zhiyong(Department of Toxicology,Tianjin Center for Disease Control and Prevention,Tianjing 300011,China)

机构地区：[1]天津市疾病预防控制中心毒理科,天津300011

出　　处：《实验动物与比较医学》2020年第5期420-425,共6页Laboratory Animal and Comparative Medicine

摘　　要：目的观察SD大鼠自发性肿瘤发生情况,为开展新药致癌试验提供背景数据。方法某项致癌试验正常对照组中4周龄SPF级SD大鼠共120只,雌雄各半,实验前观察1周,常规饲料饲养104周后实施安死术。对实验期间死亡大鼠和处死大鼠进行解剖后称量心、肺、肝、脾、肾、肾上腺、胸腺、脑、睾丸和附睾(或子宫)等器官,常规病理学取材制片后光学显微镜观察各脏器自发肿瘤的类型和发生率。结果共观察了113只SD大鼠(试验过程中损失了7只),患瘤大鼠占66.4%(75/113),其中良性肿瘤占48.7%(55/113),恶性肿瘤占17.7%(20/113)。雄性大鼠发生肿瘤49.1%(28/57),其中良性肿瘤占50.9%(26/57),恶性肿瘤占3.5%(2/57);良性肿瘤主要是垂体腺瘤(36.8%)和肾上腺髓质腺瘤(7.0%),恶性肿瘤主要是甲状腺腺癌(1.8%)和纤维肉瘤(1.8%)。雌性大鼠发生肿瘤83.9%(47/56);其中良性肿瘤占51.8%(29/56),恶性肿瘤占32.1%(18/56);良性肿瘤主要是垂体腺瘤(50.0%)、乳腺纤维腺瘤(12.5%)和乳腺腺瘤(12.5%),恶性肿瘤主要是乳腺腺癌(25.0%)和纤维肉瘤(3.6%)。结论正常饲养的2岁多的SD大鼠自发肿瘤发生率较高,且良性肿瘤发生率高于恶性肿瘤,可能与衰老、肿瘤以及个体差异有关。Objective To observe the occurrence of spontaneous tumors in SD rats,and to provide the background data for carrying out new drug induction trials.Methods In a carcinogenicity test,120 normal SD rats(SPF-grade)at 4 weeks of age were divided into male and female with the same number.All rats were observed for one week before the experiment.After 104 weeks of conventional feeding,the sedation was performed.The dead rats during the experiment and the sacrificed rats were dissected.The organs such as heart,lung,liver,spleen,kidney,adrenal gland,thymus,brain,testis and epididymis(or uterus)were weighed,and the types and incidence of spontaneous tumors in each organ were observed by an optical microscope after routine pathology.Results A total of 113 SD rats were observed(7 rats were lost during the test),the tumor-bearing rats accounted for 66.4%(75/113),of which the benign tumors accounted for 48.7%(55/113),and the malignant tumors accounted for 17.7%(20/113).Tumors occurred in 49.1%(28/57)of male rats,of which 50.9%(26/57)were benign tumors,and 3.5%(2/57)were malignant tumors;the benign tumors were mainly pituitary adenomas(36.8%)and adrenal medulla tumor(7.0%),the malignant tumors were mainly thyroid adenocarcinoma(1.8%)and fibrosarcoma(1.8%).Female rats had tumors of 83.9%(47/56),of which the benign tumors accounted for 51.8%(29/56),and the malignant tumors were 32.1%(18/56);the benign tumors were mainly pituitary adenomas(50.0%),breast fibrous glands tumors(12.5%)and breast adenomas(12.5%),the malignant tumors were mainly breast adenocarcinoma(25.0%)and fibrosarcoma(3.6%).Conclusion The incidence of spontaneous tumors was higher in more than 2 years old SD rats,and the incidence of benign tumors was higher than that of malignant tumors,which may be related to aging,tumors and individual differences.

关 键 词：自发性肿瘤 病理学观察 SD大鼠 

分 类 号：Q95-33[生物学—动物学]