酵母浸出物FP103对CHO细胞生长的影响  

Effect of extract FP103 from yeast on growth of CHO cells

作　　者：李秋霞 颜为红 贺海波 邹坤 冯旻璐 许海燕 程凡 李啸[2] LI Qiu-xia;YAN Wei-hong;HE Hai-bo;ZOU Kun;FENG Min-lu;XU Hai-yan;CHENG Fan;LI Xiao(College of Biological and Pharmaceutical Sciences&Hubei Key Laboratory of Natural Products Research and Development,China Three Gorges University,Yichang 443002,Hubei Province,China;不详)

机构地区：[1]三峡大学生物与制药学院,三峡大学天然产物研究与利用湖北省重点实验室,湖北宜昌443002 [2]安琪酵母股份有限公司,湖北宜昌443003

出　　处：《中国生物制品学杂志》2020年第9期1000-1002,1008,共4页Chinese Journal of Biologicals

基　　金：国家自然科学基金项目(21272136)。

摘　　要：目的探讨安琪酵母浸出物FP103对CHO细胞生长的影响。方法分别采用含3、1、0.125 g/L酵母浸出物FP103的DMEM/F12培养基(含1%FBS)培养CHO细胞,CCK-8法检测细胞生长情况。另将酵母浸出物FP103与表皮生长因子(epidermal growth factor,EGF)联合加入DMEM/F12培养基(含0.5%FBS)中,用其培养CHO细胞,CCK-8法检测细胞生长情况,显微镜下观察CHO细胞形态。结果1 g/L的酵母浸出物FP103可明显促进CHO细胞的生长(P<0.05)。酵母浸出物FP103与EGF联用可有效促进CHO细胞在低血清浓度下正常生长,且细胞形态正常,未见细胞悬浮不贴壁现象。结论酵母浸出物FP103可替代部分血清,为CHO细胞的生长提供所需营养。Objective To investigate the effect of extract FP103 from yeast on growth of CHO cells.Methods CHO cells were cultured in DMEM/F12 medium(containing 1%FBS)added with 3,1 and 0.125 g/L yeast extract FP103 respectively,and observed for growth by CCK-8 method.Meanwhile,extract FP103 and epidermal growth factor(EGF)were added into DMEM/F12 medium containing 0.5%FBS,with which CHO cells were cultured and observed for growth by CCK-8 method,and for morphology by microscopy.Results The yeast extract FP103 at a concentration of 1 g/L significantly promoted the growth of CHO cells(P<0.05).However,FP103 in combination with EGF effectively promoted the normal growth of CHO cells at low serum concentration,while the cells were in a normal morphology without suspension.Conclusion Yeast extract FP103 can provide necessary nutrition for the growth of CHO cells instead of partial sera.

关 键 词：安琪酵母 浸出物FP103 CHO细胞 

分 类 号：Q28[生物学—细胞生物学]