草苁蓉环烯醚萜苷联合5-氟尿嘧啶对人肝癌SMMC-7721细胞EMT的抑制作用  

Inhibitory effect of Iridoid glycosides from Boschniakia rossica combined with 5-fluorouracil on EMT in human hepatocellular carcinoma SMMC-7721 cells

作　　者：董学花 朱洁波 延光海[2] 刘莉园 全吉淑[2] 李良昌[2] 尹学哲[2] DONG Xue-hua;ZHU Jie-bo;YAN Guang-hai;LIU Li-yuan;QUAN Ji-shu;LI Liang-chang;YIN Xue-zhe(Affiliated Hospital of Yanbian University,Yanji 133000,China;Yanbian University Medical College,Yanji 133000,China)

机构地区：[1]延边大学附属医院,吉林延吉133000 [2]延边大学医学院,吉林延吉133000

出　　处：《时珍国医国药》2020年第5期1038-1042,共5页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(81760659)。

摘　　要：目的研究草苁蓉环烯醚萜苷(IGBR)联合5-氟尿嘧啶(5-Fu)对人肝癌SMMC-7721细胞EMT的影响,探讨其可能的作用机制。方法 MTT法检测细胞活力并判断联合用药的相互作用;苏木素染色观察细胞形态变化;通过细胞黏附、划痕实验检测细胞的迁移能力;Western blot检测EMT相关蛋白表达;免疫荧光检测E-cadherin和Vimentin的定位情况。结果人肝癌细胞在给药48 h后,与对照组比较,给药组(IGBR组、5-Fu组、联合组)细胞活力均降低(P<0.05),IGBR联合5-Fu具有协同作用;与对照组比较,模型组TGF-β1诱导SMMC-7721细胞数量增多,呈梭形、多边形、并集聚成团,发生凝集;与模型组比较,给药组(IGBR组、5-Fu组、联合组)细胞数量少及发生纤维样改变的细胞数少;与对照组比较,模型组细胞的黏附率、划痕愈合率增加(P<0.05),与模型组相比,给药组(IGBR组、5-Fu组、联合组)细胞黏附率、划痕迁移能力降低(P<0.05);与对照组比较,模型组MMP2、MMP7、MMP9、 Snail、Slug蛋白表达上调(P<0.05),与模型组比较,给药组MMP2、MMP7、MMP9、 Snail、Slug蛋白表达降低(P<0.05);与对照组比较,模型组E-cadherin荧光信号减弱,Vimentin荧光信号增强,与给药组(IGBR组、5-Fu组、联合组)比较,模型组E-cadherin荧光信号增强,Vimentin荧光信号减弱。结论 TGF-β1可以诱导SMMC-7721细胞发生EMT,IGBR和5-Fu可以抑制SMMC-7721细胞的迁移,抑制EMT相关机制的表达。Objective To study the effect of Iridoid glycosides from Boschniakia rossica(IGBR) combined with 5-fluorouracil(5-Fu) on EMT of human hepatocellular carcinoma SMMC-7721 cells and explore its possible mechanism. Methods MTT assay to detect cell viability and judge the interaction of combined drugs. Cell morphology was observed by hematoxylin staining. Detection of cell migration ability by cell adhesion and scratch test. Western blot was used to detect the expression of EMT-related proteins, and immunofluorescence E-cadherin and Vimentin was used to detect the location of EMT-related proteins. Results Compared with the control group, the cell viability of human hepatocellular carcinoma cells in the treatment group(IGBR group, 5-Fu group, combined group) decreased 48 hours after administration(P<0.05). IGBR combined with 5-Fu has synergistic effect. Compared with the control group, the number of SMMC-7721 cells induced by TGF-β1 in the model group was increased, fusiform, polygonal, aggregated and agglutinated;compared with the model group, the number of cells in the treatment group(IGBR group, 5-Fu group, combination group) was less and the number of cells with fibroid changes was less. Compared with the control group, the cell adhesion rate and scratch healing rate in the model group increased(P< 0.05). Compared with the model group, the cell adhesion rate and scratch migration ability of the drug group(IGBR group, 5-Fu group and combination group) decreased(P< 0.05). Compared with the control group, the expression of MMP2, MMP7, MMP9, Snail and Slug protein in the model group was up-regulated(P< 0.05). Compared with the model group, the expression of MMP2, MMP7, MMP9, Snail and Slug protein in the drug group decreased(P<0.05). Compared with the control group(IGBR group, 5-Fu group and combination group),E-cadherin fluorescence signal was weakened and Vimentin fluorescence signal was enhanced in the model group. Compared with the drug group, E-cadherin fluorescence signal was enhanced and Vimentin fluorescence s

关 键 词：草苁蓉环烯醚萜苷 5-FU 肝癌 上皮间质转化 

分 类 号：R285.5[医药卫生—中药学]