基于指纹图谱、聚类分析、主成分分析评价不同产地桑叶质量  

Quality Evaluation of Mori Folium from Different Origins Based on Fingerprint, Cluster Analysis and Principal Component Analysis

作　　者：邓明慧 路娟[1] 柴瑞平[1] 赵颖 俞月 吕欣锴 冯玉[2] 李海涛[3] 陈曦[1,3] DENG Ming-hui;LU Juan;CHAI Rui-ping;ZHAO Ying;YU Yue;LÜXin-kai;FENG Yu;LI Hai-tao;CHEN Xi(Institute of Medicinal Plant Development,Peking Union Medical College,Chinese Academy of Medical Sciences,Beijing 100193,China;Pharmacy School,Shandong University of Traditional Chinese Medicine,Jinan 250355,China;Yunnan Branch,Institute of Medicinal Plant Development,Peking Union Medical College,Chinese Academy of Medical Sciences,Jinghong 666100,China)

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所,北京100193 [2]山东中医药大学药学院,山东济南250355 [3]中国医学科学院北京协和医学院药用植物研究所云南分所,云南景洪666100

出　　处：《时珍国医国药》2020年第5期1139-1142,共4页Lishizhen Medicine and Materia Medica Research

摘　　要：目的采用UPLC建立桑叶指纹图谱,结合主成分、因子和聚类分析对不同产地桑叶综合品质进行研究,为其质量评价提供理论基础。方法 Waters BEH shield RP18色谱柱(100mm×2.1mm,1.7μm),乙腈(A)-0.1%磷酸水(B)为流动相,梯度洗脱:0~2.5min,15%~16%A;2.5~2.6min,16%~25%A;2.6~7min,25%~27%A;7~8min,27%~100%A,流速0.3mL·min^-1,柱温40℃,全波长扫描(190-400nm),波长切换(0~3 min,325 nm,检测新绿原酸、隐绿原酸、绿原酸;3~8 min,358 nm,检测芦丁、异槲皮苷、紫云英苷)。结果聚类分析较好地对不同产地的桑叶进行分类,说明不同产地桑叶在品质上存在差异。主成分、因子分析表明陕西商洛桑叶质量总体优于其他产地,3、4、6、7、8、11、12、14共有峰可作为桑叶质量评价指标成分,其中4号峰为新绿原酸,6号峰为隐绿原酸,7号峰为绿原酸,12号峰为芦丁,其余峰未知。结论通过主成分、因子和聚类分析,实现快速分析不同产地桑叶药材的质量,为桑叶整体质量控制提供了新的评价模式。Objective To establish the fingerprint of Mori Folium by UPLC, and study the comprehensive quality of Mori Folium by combining principal components, factors and cluster analysis, so as to provide theoretical basis for its quality evaluation. Methods The chromatographic column was Waters BEH shield RP18(100 mm×2.1 mm, 1.7μm), the flow phase was acetonitrile(A)-0.1% phosphoric acid water(B)with a gradient of 0~2.5 min, 15%~16%A;2.5~2.6 min,16%~25%A;2.6~7 min,25%~27%A;7~8 min, 27%~100%A, the flow rate was 0.3 mL·min^-1, the column temperature was 40 ℃, full wavelength scanning(190-400 nm), the detection wavelength was set at 325 nm for nochlorogenic acid, cryptochlorogenic acid, chlorogenic acid in the first 3 min, 358 nm for rutin, isoquercetin, astragalin during 3~8 min. Results The cluster analysis better classified Mori Folium from different producing areas, indicating that the quality of Mori Folium varies from place to place. The results of principal component analysis showed that the quality of Mori Folium in Shangluo Shanxi was better than other producing areas. The common peaks 3,4,6,7,8,11,12,14 can be used as the evaluation index components of Mori Folium quality, among which No.4 peak is neochlorogenic acid, No.6 peak is cryptochlorogenic acid, No.7 peak is chlorogenic acid, No.12 peak is rutin, and the other peaks are unknown. Conclusion By means of principal component, factors and cluster analysis, the quality of Mori Folium from different areas can be analyzed rapidly, which provides a new evaluation model for the overall quality control of Mori Folium.

关 键 词：桑叶 UPLC 指纹图谱 主成分分析 聚类分析 

分 类 号：R284.2[医药卫生—中药学]