不同产地南药益智的AFLP分析  

Analysis of AFLP for Southern Medicine Alpinia oxyphylla from Different Habitats

作　　者：苏增一 张娉 王芳 章恩硕 吴昊 高炳淼[1] SU Zeng-yi;ZHANG Pin;WANG Fang;ZHANG En-shuo;WU Hao;GAO Bing-miao(College of Pharmacy,Hainan Medical University,Haikou 571199,China)

机构地区：[1]海南医学院药学院,海南海口571199

出　　处：《时珍国医国药》2020年第5期1231-1233,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(81560611);国家级大学生创新创业训练计划项目(201611810069)。

摘　　要：目的通过对AFLP分子标记的引物筛选和聚丙烯酰胺凝胶电泳进行优化和分析,获得一套适用于南药益智的AFLP技术体系。方法采用8对引物随机配对形成64对引物,以不同产地的益智基因组DNA为模板进行AFLP-PCR扩增,再利用聚丙烯酰胺凝胶电泳分析,筛选能够鉴定益智遗传多样性的核心引物,进一步采用NTSYS软件进行聚类分析。结果从64对引物中筛选了4对引物适合于益智AFLP分析,并以E3/M7引物组合效果最佳,扩增出104条多态性带,多态性带比率为94.54%,能够鉴定不同产地益智的种质资源。结论获得适合分析不同产地益智的AFLP技术体系,为研究南药益智遗传多样性奠定基础。Objective To obtain a set of AFLP system suitable for southern medicine Alpinia oxyphylla, some primers were screened and polyacrylamide gel electrophoresis(PAGE) was used for optimization and analysis. Methods Eight pairs of primers were randomly paired to form 64 pairs of primers. AFLP-PCR was used to amplify the genomic DNA of Alpinia oxyphylla from different habitats, and then the core primers were identified by PAGE. Further, NTSYS software was used to cluster analysis. Results Four pairs of primers were selected from 64 pairs of primers for AFLP analysis. E3/M7 primer combination was the best. 104 polymorphic bands were amplified, the ratio of polymorphic bands was 94.54%, which could identify the germplasm resources of Alpinia oxyphylla from different habitats.Conclusion The AFLP technology system suitable for the analysis of Alpinia oxyphylla from different habitats was obtained, which laid a foundation for the study of genetic diversity of Alpinia oxyphylla.

关 键 词：益智 AFLP 引物筛选 聚丙烯酰胺凝胶电泳 聚类分析 

分 类 号：S567[农业科学—中草药栽培] R2-031[农业科学—作物学]