鼠尾草酚通过PPARγ信号通路调节膀胱癌T24细胞增殖及侵袭机制研究  

Study on Mechanism of Carnosol Regulating Proliferation and Invasion of Bladder Cancer T24 Cells by PPARγ Signal Pathway

作　　者：倪建华 朱江波[1] 殷国林 章庆华[1] NI Jianhua;ZHU Jiangbo;YIN Guolin;ZHANG Qinghua

机构地区：[1]台州市第一人民医院泌尿外科,浙江台州318020

出　　处：《新中医》2020年第19期4-8,共5页New Chinese Medicine

摘　　要：目的:探讨鼠尾草酚调节膀胱癌T24细胞增殖及侵袭的分子机制。方法:将膀胱癌T24细胞分为T24细胞组、5-氟尿嘧啶组及鼠尾草酚低、高剂量组(低、高剂量组)。5-氟尿嘧啶组加入50.0μg/mL的5-氟尿嘧啶,低、高剂量组分别加入50.0μg/mL、100.0μg/mL的鼠尾草酚,培养72 h。测定各组细胞增殖情况、克隆形成数目、侵袭迁移水平、凋亡水平及过氧化物酶体增殖物激活受体γ(PPARγ)、β-连环蛋白(β-catenin) mRNA与蛋白水平。结果:与T24细胞组比较,其余各组细胞凋亡率、PPARγm RNA及蛋白表达水平升高(P<0.05),OD值、存活率、细胞克隆形成数目、穿膜数、β-catenin mRNA及蛋白表达水平降低(P<0.05)。与5-氟尿嘧啶组比较,低剂量组细胞凋亡率、PPARγmRNA及蛋白表达水平降低(P<0.05),OD值、存活率、细胞克隆形成数目、穿膜数、β-catenin mRNA及蛋白表达水平升高(P<0.05);高剂量组细胞凋亡率、PPARγmRNA及蛋白表达水平升高(P<0.05),OD值、存活率、细胞克隆形成数目、穿膜数、β-catenin m RNA及蛋白表达水平降低(P<0.05)。与低剂量组比较,鼠尾草酚高剂量组细胞凋亡率、PPARγmRNA及蛋白表达水平升高(P<0.05),OD值、存活率、细胞克隆形成数目、穿膜数、β-catenin mRNA及蛋白表达水平降低(P<0.05)。结论:鼠尾草酚能抑制膀胱癌T24细胞增殖、侵袭迁移,促进膀胱癌T24细胞凋亡;其机制可能与鼠尾草酚能促进PPARγmRNA及蛋白表达,激活PPARγ信号通路进而抑制β-catenin mRNA和蛋白表达有关。Objective: To investigate the molecular mechanism of carnosol regulating the proliferation and invasion of bladder cancer T24 cells. Methods:Bladder cancer T24 cells were respectively divided into the T24 cell group,the5-fluorouracil group and the carnosol groups with low and high dose(the low-dose group and the high-dose group).The 5-fluorouracil group was given 50.0 μg/mL of 5-fluorouracil,and the low-dose and high-dose groups were given 50.0 μg/mL and 100.0 μg/mL of carnosol respectively. All groups were cultured for 72 hours. Cell proliferation,the number of colony formation as well as the levels of invasion and migration, apoptosis, peroxisome proliferator activated receptor γ(PPARγ),β-catenin m RNA and protein in each group were measured. Results:Compared with those in the T24 cell group,the apoptosis rates and the levels of PPARγ m RNA and protein expressions in the other groups were increased(P<0.05);the OD value,survival rate,the number of cell clone formation and cell membrane penetration,and the levels ofβ-catenin mRNA and protein expressions were decreased(P<0.05). Compared with those in the 5-fluorouracil group,the apoptosis rate and the levels of PPARγ mRNA and protein expressions in the low-dose group were decreased(P<0.05),and the OD value,survival rate,the numbers of cell clone formation and cell membrane penetration,and the levels of β-catenin mRNA and protein expressions were increased(P<0.05);the apoptosis rate and the levels of PPARγ mRNA and protein expressions in the high-dose group were increased(P<0.05),and the OD value,survival rate,the number of cell clone formation and cell membrane penetration,and the levels of β-catenin mRNA and protein expressions were decreased(P<0.05). Compared with those in the low-dose group, the apoptosis rate and the levels of PPARγ mRNA and protein expressions in the high-dose group were increased(P<0.05),and the OD value,survival rate,the number of cell clone formation and cell membrane penetration,and the levels of β-catenin mRNA and protein e

关 键 词：膀胱癌 鼠尾草酚 PPARγ信号通路 增殖 侵袭 细胞实验 

分 类 号：R285.5[医药卫生—中药学]