自体来源脂肪干细胞构建工程化心肌补片修补大鼠左心室室壁瘤的研究  

Construction of engineering heart tissues using autologous adipose derived stem cells for repairing left ventricular aneurysm

作　　者：吴华 钟胜[1,2] 谷彪 王旗[1,2] 吴清泉 WU Hua;ZHONG Sheng;GU Biao;WANG Qi;WU Qingquan(Department of Thoracic Surgery,The Affiliated Huaian No.1 People's Hospital of Nanjing Medical University,Huaian 223300,China)

机构地区：[1]南京医科大学附属淮安第一医院胸心外科,223300 [2]淮安市第一人民医院胸心外科

出　　处：《心肺血管病杂志》2020年第9期1127-1133,共7页Journal of Cardiovascular and Pulmonary Diseases

摘　　要：目的:旨在比较自体来源脂肪干细胞(ADSCs)与骨髓间充质干细胞(BMSCs)构建工程化心肌组织(EHTs)修补大鼠左心室室壁瘤,为EHTs提供合适的种子细胞。方法:实验共分四组(n=6),分别为年轻脂肪干细胞组(YA),年轻骨髓干细胞组(YB),老年脂肪干细胞组(OA),老年骨髓干细胞组(OB)。于左胸第三肋间结扎大鼠前降支,2周后行心脏功能检测,选取心脏梗死面积为25%~30%的大鼠进行实验。将ADSCs与BMSCs(1.0×10~6/片)种植于经EDC(24 mg/mL)及NHS(60 mg/mL)活化、同时共价结合VEGF(1μg/mL)及bFGF(1μg/mL)的胶原蛋白补片,3 d后行左心室室壁瘤修补。术后7、14及28 d测定心脏超声检测左心室室壁瘤重建术后心脏功能;固定距离(35 cm)下测定补片面积大小及补片厚度;同时测定了新生血管生成情况及内皮细胞覆盖。结果:EHTs植入体内28 d后,OA组大鼠心脏功能在EF值、FS值高于OB组,OA组LVIDd及LVIDs值低于OB组,而YA组与YB组,差异无统计学意义(P>0.05)。此外,OA组大鼠心脏补片面积低于OB组(P<0.05),OA组补片厚度高于OB组(P<0.05),而YA组与YB组差异无统计学意义(P>0.05)。同时,在细胞存活及新生血管生成方面,OA组明显高于OB组(P<0.05),而YA组与YB组,差异无统计学意义(P>0.05)。结论:老年患者来源的ADSCs更加适合作为种子细胞构建EHTs进行细胞移植。Objective:This study was aimed to compare the difference between engineering heart tissues constructed with adipose derived stem cells(ADSCs)and bone marrow stem cells(BMSCs)to repair left ventricular aneurysm and to select an optimal candidate for cell transplant.Methods:This study was divided into four groups:YA group,YB group,OA group and OB groups(n=6).Left descending artery was ligated through 3rd intercostal to form left ventricular aneurysm.2 weeks later,cardiac function was measured to select rats,of which infarct size was between 25%and 30%.ADSCs and BMSCs(1.0×106/scaffold)were implanted onto the scaffold which was activated using EDC(24 mg/mL)and NHS(60 mg/mL),covalently combined with VEGF(1μg/mL)and bFGF(1μg/mL).3 days after incubation,EHTs were implanted into the heart.In vivo,cardiac function,area and thickness of patches and angionesis were assessed 28 days after EHTs implantation.Results:In vivo,28 days after implantation,EF value and FS value were higher in OA group than OB group(P<0.05);Left ventricular diastolic internal diameter(LVIDd)and left ventricular systolic internal diameter(LVIDs)were lower in OA group than OB group(P<0.05).However,there was no significant difference between YA group and YB group(P>0.05).Meanwhile,cell survival and angionesis were higher in OA group than OB group(P<0.05),and no significant differences were observed between YA group and YB group(P>0.05).Conclusions:Adipose derived stem cells from elder patients were more optimal for constructing EHTs.

关 键 词：脂肪干细胞 骨髓间充质干细胞 左心室室壁瘤 

分 类 号：R54[医药卫生—心血管疾病]