女贞子与酒女贞子HPLC指纹图谱对比研究  

Comparative study on HPLC fingerprints between crude and processed Ligustri Lucidi Fructus

作　　者：张浩杰 李焕茹 冯志毅[1] 李柯 胡雁萍 冯素香[1,2,3] ZHANG Hao-jie;LI Huan-ru;FENG Zhi-yi;LI Ke;HU Yan-ping;FENC Su-xiang(Henan University of Chinese Medicine,Zhengahou 450046,China;Co-construction Collaborative Innoation Center for Chinese Medicine and Respiratory Diseases by Henan&Education Ministry of China,Zhengzhou 450046,China;Zhengzhou Key Laboratory of Chinese Medicine Quality Control and Eraluation,Zhengzhou 450046,China)

机构地区：[1]河南中医药大学,河南郑州450046 [2]呼吸疾病中医药防治省部共建协同创新中心,河南郑州450046 [3]郑州市中药质量控制与评价重点实验室,河南郑州450046

出　　处：《中国中药杂志》2020年第16期3871-3876,共6页China Journal of Chinese Materia Medica

基　　金：河南省科技攻关项目(142102310280)。

摘　　要：建立女贞子及酒女贞子的HPLC指纹图谱,并结合相似度计算和化学模式识别方法,评价女贞子、酒女贞子质量。采用Syncronis C18(4.6 mm×250 mm,5μm)色谱柱,以乙腈-0.1%磷酸水溶液梯度洗脱,检测波长为280 nm。采用HPLC对22批不同产地女贞子及酒女贞子进行测定,采用"中药色谱指纹图谱相似度评价系统(2012版)"计算相似度,运用聚类分析(CA)、主成分分析(PCA)和最小偏二乘法-判别分析(PLS-DA)对其进行模式识别研究。建立了22批女贞子及酒女贞子的HPLC指纹图谱,相似度均在0.9~1.0,通过CA,PCA,PLS-DA可将女贞子与酒女贞子完全区分开来,通过PLS-DA分析可知羟基酪醇、酪醇、特女贞苷、橄榄苦苷等11个成分是导致二者差异的主要标志成分。建立的指纹图谱方法稳定可靠,可为女贞子、酒女贞子质量控制提供方法依据,同时结合化学模式识别研究有助于女贞子炮制前后整体质量控制及质量评价。To establish high performance liquid chromatography(HPLC)fingerprints for crude and processed Ligustri Lucidi Fructus,and to evaluate their quality through the similarity calculation and chemical pattern recognition.The separation was performed with Syncronis C18 column(4.6 mm×250 mm,5μm),with acetonitrile(A)and 0.1%phosphoric acid solution(B)as the mobile phase for gradient elution,and a detection wavelength of 280 nm.HPLC was used to detect 22 batches of crude and processed Ligustri Lucidi Fructus,and the Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(2012 Edition)was used to evaluate the similarity among 22 batches.The research on pattern recognition was conducted with cluster analysis(CA),principal component analysis(PCA),and partial least squares discriminate analysis(PLS-DA).HPLC fingerprints of crude and processed Ligustri Lucidi Fructus were established,with similarity ranging from 0.9 to 1.0.The crude and processed Ligustri Lucidi Fructus can be obviously distinguished by using CA,PCA and PLS-DA.According to the results of PLS-DA,11 constituents including hydroxytyrosol,tyrosol,specnuezhenide and oleuropein were the main marker components leading to the difference.The established fingerprint method is stable and reliable,and can provide method basis for quality control of crude and processed Ligustri Lucidi Fructus.Chemical pattern recognition is proved to be helpful in comprehensive quality control and evaluation of Ligustri Lucidi Fructus before and after the process.

关 键 词：女贞子 指纹图谱 化学模式识别 质量评价 

分 类 号：R284.1[医药卫生—中药学]