屠宰环节禽产品中金黄色葡萄球菌污染状况调查  

Investigation on Staphylococcus aureus Pollution in Slaughtered Poultry Products

作　　者：谢建华[1] 叶洁莹 王棋 梁望旺[1] 杨泽林[1] 熊仲良[1] 曾政[1] XIE Jian-hua;YE Jie-ying;WANG Qi(Chongqing Animal Disease Prevention and Control Center,Chongqing 401120)

机构地区：[1]重庆市动物疫病预防控制中心,重庆401120

出　　处：《安徽农业科学》2020年第20期163-165,共3页Journal of Anhui Agricultural Sciences

基　　金：社会民生类重点研发项目(cstc2018jscx-mszdX0049);重庆市技术创新与应用发展专项(cstc2019jscx-msxmX0370)。

摘　　要：[目的]了解重庆市家禽屠宰环节金黄色葡萄球菌的污染情况,为防控金黄色葡萄球菌食源性疾病的发生提供参考依据。[方法]按照GB 4789.10—2016《食品安全国家标准食品微生物学检验金黄色葡萄球菌检验》对重庆市屠宰环节400份样品进行检测,同时随机选择40株金黄色葡萄球菌进行药敏试验和致病性分析。[结果]屠宰环节金黄色葡萄球菌污染程度达59.25%,秋季较夏季污染程度重,为67.00%,胴体比环境污染重,为65.71%,烫洗煺毛和净膛环节污染风险较重均达60%以上。40株分离株中毒素基因检出率为65.00%,总耐药率为95.00%,主要对青霉素、四环素、红霉素和克林霉素耐药;70.00%的菌株同时对3种以上抗生素耐药。[结论]重庆市家禽屠宰环节金黄色葡萄球菌污染较重,部分分离株存在多个毒素基因和多重耐药现象,值得高度重视。[Objective]To understand the pollution of Staphylococcus aureus in slaughtered poultry products in Chongqing.[Method]According to GB 4789.10-2016 National Food Safety Standard-Food Microbiological Examination S.aureus,a total of 400 samples in Chongqing,40 strains of S.aureus were randomly selected for drug sensitivity test and pathogenicity analysis.[Result]S.aureus pollution reached 59.25%.Autumn was more polluted than summer,67.00%.Carcass was more polluted than environment,65.71%.The pollution risks in ironing and cleaning was more than 60%.In 40 isolates,the detected rate of toxin gene was 65.00%,and the average drug resistance rate was 95.00%.The S.aureus isolates were highly resistant to penicillin,tetracycline,erythromycin and clindamycin,70.00%strains displayed multi-drug resistance.[Conclusion]The pollution of S.aureus in slaughtered poultry products in Chongqing is serious,some isolates had multiple toxin genes and multi drug resistance,which should be paid more attention to.

关 键 词：金黄色葡萄球菌 禽产品 细菌污染 

分 类 号：S852.61[农业科学—基础兽医学]