溴甲酚绿法与溴甲酚紫法对血清糖化清蛋白水平检测的影响  

Comparative Study on the Bromocresol Green and Bromocresol Purple Method for the Detection of Glycated Albumin

作　　者：蔡德佩 王聪[1] 王晓倩 胡健[1] 王晓琴[1] CAI De-pei;WANG Cong;WANG Xiao-qian;HU Jian;WANG Xiao-qin(Department of Clinical Laboratory,the First Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710061,China)

机构地区：[1]西安交通大学第一附属医院检验科,西安710061

出　　处：《现代检验医学杂志》2020年第5期128-130,172,共4页Journal of Modern Laboratory Medicine

基　　金：西安交通大学第一附属医院临床研究课题(XJTU1AF-CRF-2016-019)。

摘　　要：目的探讨溴甲酚绿(bromocresol green,BCG)法和溴甲酚紫(bromocresol purple,BCP)法测定血清清蛋白(albumin,Alb)对糖化清蛋白(glycated albumin,GA)的结果影响。方法回顾性收集2018年1~12月于西安交通大学第一附属医院就诊患者溴甲酚绿法清蛋白(AlbBCG)和溴甲酚紫法清蛋白(AlbBCP)的检验数据,分别计算溴甲酚绿法糖化清蛋白(GABCG)和溴甲酚紫法糖化清蛋白(GABCP),并比较两种方法所测得的结果是否存在差异。结果随着患者体内Alb水平的降低,两种方法测得的Alb值和GA值出现显著性差异。当Alb<35g/L时,BCG法比BCP法Alb测定的Alb值略高,约高2.18g/L;BCG法比BCP法测定的GA值略低,约低1.01%。当Alb≥35g/L时,两种方法测定GA值无显著统计学差异(t=2.0,P=0.05)。两种检测方法计算GA结果一致性均较好,但当Alb≥35g/L时,两种方法对于GA检测一致性更高。结论在糖化清蛋白检测中,若患者血清中Alb较低,BCG法非特异性反应增加,导致所测的GA值低于BCP法所测得的GA值,可能低估患者GA水平。因此,BCP法更适合检测伴有低蛋白血症糖尿病患者的GA值。Objective To compare bromocresol green(BCG)method and bromocresol purple(BCP)method for calculation of glycated albumin(glycated albumin,GA)by detecting serum albumin(Alb).Methods The data from the First Affiliated Hospital of Xi’an Jiaotong University from January to December 2018 were retrospectively collected and statistically analyzed.Results At the low level of the Alb,significant inconsistencies were observed between the two methods for detection of Alb and GA.When Alb<35g/L,Alb detected by BCG method(AlbBCG)was about 2.18 g/L higher than that detected by BCP method(AlbBCP),and the corresponding GA values detected by BCG method(GABCG)were about 1.01%lower than that detected by BCP(GABCP).When Alb>35g/L,there was no significant difference in GA values detected by the two methods(t=2.0,P>0.05).Alb values detected by the two methods showed high consistency,however,the two methods had higher consistency for GA detection when Alb≥35g/L.Conclusion For the detection of glycated albumin,GABCG might be lower than the actual GA level in patients when Alb levels are low in patients,so recommend BCP method for detection of GA in diabetics with hypoproteinemia.

关 键 词：溴甲酚绿 溴甲酚紫 清蛋白 糖化清蛋白 

分 类 号：R446.112[医药卫生—诊断学]