孕哺期十溴联苯醚暴露对子鼠神经甾体水平和学习记忆能力的影响  

Effects of decabromodiphenyl ether exposure during pregnant and lactating periods on neurosteroid and learning and memory ability of offspring mice

作　　者：蒋荣娟 赵超超 王程强 宋家乐 钱波 JIANG Rong-juan;ZHAO Chao-chao;WANG Cheng-qiang;SONG Jia-le;QIAN Bo(Department of Ultrasound,Affiliated Hospital of Guilin Medical University,Guilin,Guangxi 541000,China;Department of Environmental Health and Occupational Medicine,School of Public Health,Guilin Medical University,Guilin,Guangxi 541000,China)

机构地区：[1]桂林医学院附属医院超声科,广西桂林541000 [2]桂林医学院公共卫生学院环境卫生与职业医学教研室,广西桂林541000

出　　处：《环境与职业医学》2020年第9期909-914,共6页Journal of Environmental and Occupational Medicine

基　　金：广西高校中青年教师基础能力提升项目(2018KY0423);广西高等学校千名中青年骨干教师培育计划资助(桂教人[2018]18号);人社部“高层次留学人才回国资助计划”(人社厅函[2019]160号);桂林医学院引进人才科研启动基金(04010150001)。

摘　　要：[背景]十溴联苯醚(BDE209)的神经发育毒性机制尚不清楚,研究资料提示神经甾体紊乱可能是其诱发神经发育毒性的重要原因。[目的]探索BDE209对神经甾体水平的影响,以及神经甾体紊乱在BDE209诱发神经发育毒性中的作用。[方法]60只ICR雌鼠随机分为0、225、900 mg·kg^-1 BDE209染毒组,受孕成功后经口灌胃母鼠BDE209至子鼠出生后第21天。每个剂量组选取24只子鼠,通过Morris水迷宫实验检测子鼠学习记忆能力,使用酶联免疫吸附试验测定子鼠大脑和血清孕酮(PROG)、3α-5α四氢孕酮(3α-5α-THP)、脱氢表雄酮(DHEA)、孕烯醇酮(PREG)水平,采用JC-1荧光探针检测子鼠海马线粒体膜电位。[结果]水迷宫实验结果显示:各组逃避潜伏期随着训练天数增加均呈下降趋势,与训练第1天相比,训练第4天,对照组、225 mg·kg^-1染毒组和900 mg·kg^-1染毒组逃避潜伏期分别下降51.34%,27.20%和16.87%。水迷宫实验第5天,225、900 mg·kg^-1染毒组象限穿越次数与对照组相比分别下降51.81%和67.47%,象限停留时间分别下降48.45%和77.47%,平台穿越次数分别下降64.29%和75.00%(均P<0.05)。大脑神经甾体检测结果显示,与对照组相比:225、900 mg·kg^-1染毒组PROG分别下降36.51%和69.28%,3α-5α-THP分别下降31.84%和57.43%,DHEA分别下降31.13%和60.15%,PREG分别下降31.17%和68.07%(均P<0.05)。血清神经甾体检测结果显示,与对照组相比:225、900 mg·kg^-1染毒组PROG分别下降29.49%和63.44%,3α-5α-THP分别下降25.12%和53.71%,DHEA分别下降29.09%和56.97%,PREG分别下降25.39%和47.07%(均P<0.05)。海马线粒体膜电位检测结果显示:225、900 mg·kg^-1染毒组线粒体膜电位与对照组相比分别下降10.06%和25.58%(均P<0.05)。[结论]BDE209具有神经发育毒性,孕哺期BDE209染毒可能通过诱发神经甾体紊乱从而抑制子鼠学习记忆能力。[Background]Decabromodiphenyl ether(BDE209)has neurodevelopmental toxicity,but the associated mechanism is not clear.Previous studies have shown that neurosteroid disorder may be one of the main reasons.[Objective]This experiment explores the effect of BDE209 on neurosteroid levels,and the role of neurosteroid disorder in neurodevelopmental toxicity induced by BDE209.[Methods]Sixty female ICR mice were randomly divided into 0,225,and 900 mg·kg^-1 BDE209 exposure groups.After conception,the pregnant mice were administered with BDE209 by oral gavage to 21 days after offspring birth.Twenty-four offspring mice of each group were selected,their learning and memory ability was tested by Morris water maze,their levels of progesterone(PROG),3α-5α-tetrahydroprogesterone(3α-5α-THP),dehydroepiandrosterone(DHEA),and pregnenolone(PREG)in brain and serum were detected by enzyme-linked immunosorbent assay,and mitochondrial membrane potential in hippocampus was detected using a JC-1 fluorescence probe.[Results]The water maze experiment results showed that the escape latencies of the designed groups decreased with prolonged training days,and the decreases on day 4 versus day 1 of the control group,the 225 mg·kg^-1 exposure group,and the 900 mg·kg^-1 exposure group were 51.34%,27.20%,and 16.87%,respectively.On day 5 of the water maze experiment,compared with the control group,the number of quadrant crossing of the 225 and 900 mg·kg^-1 exposure groups were decreased by 51.81%and 67.47%,the quadrant staying time by 48.45%and 77.47%,and the number of platform crossing by 64.29%and 75.00%,respectively(P<0.05).The brain neurosteroid test results showed that compared with the control group,the PROG of the 225 and 900 mg·kg^-1 exposure groups were decreased by 36.51%and 69.28%,the 3α-5α-THP by 31.84%and 57.43%,the DHEA by 31.13%and 60.15%,and PREG by 31.17%and 68.07%,respectively(P<0.05).The serum neurosteroid test results showed that compared with the control group,the PROG of the 225 and 900 mg·kg^-1 exposure groups were de

关 键 词：十溴联苯醚 神经发育毒性 神经甾体紊乱 学习记忆 

分 类 号：R114[医药卫生—卫生毒理学]