彩云木叶化学成分及其抗炎活性研究  

Chemical constituents from the leaves of Synadenium grantii and their anti-inflammatory activity

作　　者：阳航 邹情雅 陈宇 路艳霞 刘梦元 李路军[1,3,4] PaulW.Paré YANG Hang;ZHOU Qing-ya;CHEN Yu;LU Yan-xia;LIU Meng-yuan;LI Lu-jun;Paul W.Paré(National & Local Joint Engineering Research Center of High-throughput Drug Screening Technology,State Key Laboratory of Biocatalysis and Enzyme Engineering,Hubei Key Laboratory of Biotechnology of Chinese Traditional Medicine,Hubei University,Wuhan 430062,China;Department of Chemistry & Biochemistry,Texas Tech University,Lubbock 79409,USA;Gansu Herbal Medicine Planting Co.,Ltd.,Lanzhou 730102,China;Shenzhen Institue of Geriatrice,Shenzhen 518020,China)

机构地区：[1]药物高通量筛选国家与地方联合工程研究中心,生物催化与酶工程国家重点实验室,中药生物技术湖北省重点实验室,湖北大学,武汉430062 [2]美国德州理工大学化学与生物化学系,拉伯克79409 [3]甘肃百草中药材种植有限公司,兰州730102 [4]深圳市老年医学研究所,深圳518020

出　　处：《天然产物研究与开发》2020年第10期1698-1703,共6页Natural Product Research and Development

基　　金：国家自然科学基金(81560703);深圳市基础研究计划自由探索项目(JCYJ20170306171157738);药物高通量筛选技术国家地方联合工程研究中心开放基金(M20181007);药学“荆楚卓越人才”协同育人计划大学生创新训练计划(M20191002)。

摘　　要：为研究彩云木Synadenium grantii叶的化学成分及部分化合物体外抗炎活性,本研究采用硅胶、Sephadex LH-20等多种柱色谱进行分离纯化,通过理化性质和NMR等波谱数据鉴定化合物的结构,并对部分化合物进行抑制脂多糖诱导RAW 264.7细胞释放NO活性的测定。从彩云木叶二氯甲烷-甲醇(1∶1)提取物中共分离得到了10个化合物,分别鉴定为日尔曼醇(1)、大戟醇(2)、正二十八烷醇(3)、β-谷甾醇(4)、豆甾醇(5)、ingol-7,8,12-triacetate-3-phenylacetate(6)、山柰酚-3-O-α-L-吡喃鼠李糖苷(7)、胡萝卜苷(8)、3,4,3′-三甲氧基鞣花酸-4′-O-β-D-吡喃葡萄糖苷(9)、山奈酚-3-O-β-D-吡喃葡萄糖苷(10)。其中3和6~10为首次从该种植物中分离得到,3、6、8和9为首次从聚苞大戟属植物中分离得到,9抑制LPS诱导RAW 264.7细胞释放NO的IC50值为12.0±0.9μM,显示较好的体外抗炎活性。To investigate the chemical constituents from the leaves of Synadenium grantii and their anti-inflammatory activities in vitro,various column chromatography techniques such as column chromatography on silica gel,Sephadex LH-20 were used to isolate and purify compounds and their structures were identified by spectral data including NMR and physicochemical properties.Some compounds were evaluated for their inhibitory effects on lipopolysaccharide-induced nitric oxide production in RAW264.7 cells.Ten compounds were isolated and identified as germanicol(1),euphol(2),1-octacosanol(3),β-sitosterol(4),stigmasterol(5),ingol 7,8,12-triacetate-3-phenylacetate(6),kaempferol-3-O-α-L-rhamnpyranoside(7),daucosterol(8),3,3,4′-tri-O-methylellagic acid 4′-O-β-D-glucopyranoside(9),kaempferol-3-O-β-D-glucopyranoside(10).Compounds 3 and 6-10 were isolated from the species for the first time,among which compounds 3,6,8 and 9 were isolated from the plants in genus Synadenium for the first time.Compound 9 shows good anti-inflammatory activity in vitro with an IC50 values of 12.0±0.9μM.

关 键 词：彩云木 化学成分 ingol-7 8 12-triacetate 3-phenylacetate 抗炎活性 

分 类 号：R284.2[医药卫生—中药学]