蜂胶黄酮和酚酸类成分的大孔树脂富集纯化及其吸附行为研究  

Study on the purification of flavonoids and phenolic acids from propolis by macroporous resins and its adsorption performance

作　　者：赖伟娜 欧彩诗 袁敏 何健 李坤平 LAI Weina;OU Caishi;YUAN Min;HE Jian;LI Kunping(School of Pharmacy,Guangdong Pharmaceutical University,Guangzhou,510006,China;Chinese Medicine Research Institute,Guangdong Pharmaceutical University,Guangzhou,510006,China;Guangdong Key Laboratory for Metabolic Diseases Prevention and Treatment of TCM,Guangzhou 510006,China;BY-HEALTH Co.Ltd,Guangzhou 511436,China)

机构地区：[1]广东药科大学药学院 [2]广东药科大学中医药研究院,广东广州510006 [3]广东省代谢性疾病中医药防治重点实验室,广东广州510006 [4]汤臣倍健股份有限公司,广东广州511436

出　　处：《广东药科大学学报》2020年第5期593-598,共6页Journal of Guangdong Pharmaceutical University

基　　金：教育部留学回国人员科研启动基金(教外司留[2015]1098号);汤臣倍健科研合作项目(C01201903260013)。

摘　　要：目的开发一种利用大孔树脂富集纯化蜂胶中黄酮和酚酸类化合物的方法,并探讨其吸附过程的热力学和动力学特征。方法以芦丁为对照品,分光光度法测定总黄酮含量;以没食子酸为对照品,Folin-Ciocalteu法测定总酚酸含量。根据树脂的吸附量和解吸率,从D101、LSA-21、LSA-40、LSA-900A、LX-32、LX-60、LX-98、XDA-8G和FPA-98Cl等9种常用树脂中筛选出合适的树脂,并进行吸附热力学及动力学分析。结果FPA-98Cl树脂对蜂胶总黄酮及总酚酸的静态吸附量分别达到了80.72 mg/g和91.53 mg/g,解吸率分别为78.4%和73.4%,在上述9种树脂中吸附分离性能最好。热力学分析表明,蜂胶总黄酮在FPA-98Cl树脂上的吸附过程符合Langmuir等温线模型,蜂胶总酚酸的吸附过程符合Freundlich吸附等温线方程。动力学过程分析表明,蜂胶总黄酮在FPA-98Cl树脂上的吸附行为符合Weber-Morris颗粒内扩散方程,而Mckay准二级动力学方程更适合描述蜂胶总酚酸的吸附行为。结论大孔树脂用于富集纯化蜂胶中的黄酮和酚酸类成分是可行的,FPA-98Cl树脂是较好的选择;上述吸附热力学和动力学实验结果为后续工艺开发提供了参考。Objective To develop a novel method for the purification of the flavonoids and phenolic acids of propolis with macroporous resins,and investigate their adsorption behaviour characteristics.Methods Rutin was used as a reference for spectrophotometric determination of total flavonoids,and gallic acid was used as a reference for determination of total phenolic acids by Folin-Ciocalteu method.According to the adsorption amount and desorption rate,the best suitable resin was selected from nine usually used macroporous resins,namely,D101、LSA-21、LSA-40、LSA-900A、LX-32、LX-60、LX-98、XDA-8G and FPA-98Cl.The adsorption thermodynamics and kinetics analysis were carried out.Results For FPA-98Cl resin,the static adsorption amounts of total flavonoids and total phenolic acids were 80.72 mg/g and 91.53 mg/g,and the desorption rates were 78.4%and 73.4%,respectively.FPA-98Cl was the suitable resin for the enrichment and purification of flavonoids and phenolic acids in propolis.The adsorption process of total flavones in propolis on FPA-98Cl resin conformed to the Langmuir isotherm model,whereas the adsorption process of total phenolic acids in propolis was more consistent with the Freundlich adsorption isotherm equation.Meanwhile,the Weber-Morris intra-particle diffusion equation could describe the adsorption behaviour of total flavonoids in propolis on FPA-98Cl resin,and the Mckay quasi-second-order kinetic equation was more suitable to describe the adsorption behaviour of total phenolic acid in propolis on FPA-98Cl resin.Conclusion It is feasible to enrich and purify flavonoids and phenolics in propolis by macroporous resin,FPA-98Cl is a better choice.The experimental results of adsorption thermodynamics and kinetics provide a reference for the subsequent process development.

关 键 词：蜂胶 黄酮类化合物 酚酸类化合物 大孔树脂 吸附动力学 

分 类 号：R283.2[医药卫生—中药学]