N烷基化对吖啶橙光谱性质的影响  

Effect of N-alkylation on Spectral Properties of Acridine Orange

作　　者：冯志明[1] 伍斌[1] 冯振南 FENG Zhi-ming;WU Bin;FENG Zhen-nan(College of Chemistry and Chemical Engineering,Hunan Normal University,Hunan Changsha 410081;Hainan Medical College,Hainan Haikou 570102,China)

机构地区：[1]湖南师范大学化学化工学院,湖南长沙410081 [2]海南医学院,海南海口570102

出　　处：《广州化工》2020年第20期80-81,共2页GuangZhou Chemical Industry

摘　　要：吖啶橙衍生物在细胞生物染色等领域应用广泛。为探讨吖啶橙烷基化对其紫外光谱和荧光光谱的影响以及为筛选不同细胞通透性荧光染料提供依据,以吖啶橙为原料,通过氮烷基化合成了N-甲基吖啶橙(MAO)、N-乙基吖啶橙(EAO)、N-丙基吖啶橙(PAO)、N-丁基吖啶橙(BAO)N-戊基吖啶橙(AAO)和N-戊基吖啶橙(AAO)。N-烷基吖啶橙在496 nm和470 nm处有较强的特征吸收。pH对N-烷基吖啶橙的荧光光谱影响较大,pH=7.45时荧光强度最高。吖啶橙烷基化基本保持了母体优异的荧光特性。Acridine orange derivatives are widely used in cell biological staining and other fields.In order to explore the influence of acridine orange alkylation on its UV and fluorescence spectra and provide the basis for screening different cell permeability fluorescent dyes,N-methylacridine(MAO),N-ethylacridine(EAO),N-propylacridine(PAO),Nbutylacridine(BAO),N-amylacridine(AAO)and N-nonylacridine(AAO)were synthesized from acridine orange with nitrogen alkylation.N-alkyl acridine orange had strong characteristic absorption at 496 nm.Fluorescence pH had a significant influence on the fluorescence spectrum of n-nonylacridine orange,and the fluorescence intensity was the highest at pH 7.45.The alkylation of acridine orange maintained the excellent fluorescence characteristics of their parent.

关 键 词：吖啶橙 烷基化 光谱性质 

分 类 号：O621.22[理学—有机化学]