复方山药提取物制剂增强免疫力功能实验研究  

Experimental Study on Enhancing Immune Function of Compound Common Yam Rhizome Extract Preparation

作　　者：陈垚[1] CHEN Yao(Department of Clinical Nutrition,the People’s Hospital of Rizhao,Rizhao 276800,China)

机构地区：[1]日照市人民医院临床营养科,山东日照276800

出　　处：《中西医结合研究》2020年第5期313-318,共6页Research of Integrated Traditional Chinese and Western Medicine

摘　　要：目的观察复方山药提取物制剂对小鼠增强免疫力功能的影响。方法将160只实验小鼠随机分为Ⅰ~Ⅳ组,每大组40只。每大组再细分溶媒对照组和复方山药提取物制剂低(200 mg/kg·bw)、中(400 mg/kg·bw)、高(1200 mg/kg·bw)三个剂量小组,每小组10只。小鼠连续经口灌胃30 d后,按《保健食品检验与评价技术规范》中增强免疫力功能的检验方法,测定其细胞免疫、体液免疫、单核-巨噬细胞吞噬功能指标及NK细胞活性。结果高剂量组小鼠足跖肿胀度、抗体生成细胞数、NK细胞活性、吞噬鸡红细胞的吞噬指数均显著高于溶媒对照组(P<0.05),中剂量组小鼠NK细胞活性、吞噬鸡红细胞的吞噬指数显著高于溶媒对照组(P<0.01)。与溶媒对照组比较,各剂量组小鼠体重增长、胸腺/体重比值、脾脏/体重比值、血清溶血素水平、淋巴细胞增殖能力、单核-巨噬细胞碳廓清能力差异均无统计学意义(P>0.05)。结论复方山药提取物制剂能显著提高实验小鼠迟发型变态反应能力、抗体生成细胞数、巨噬细胞吞噬鸡红细胞的能力和NK细胞活性,具有增强免疫力功能。Objective To observe the effect of compound common yam rhizome extract preparation on enhancing immune function of mice.Methods A total of 160 experimental mice were randomly divided into groupsⅠ~Ⅳ,with 40 mice in each part.Each experiment part was randomly divided into four groups,the solvent control group and the compound yam extract preparation groups:low-dose(200 mg/kg·bw),medium-dose(400 mg/kg·bw)and high-dose(1200 mg/kg·bw),with 10 mice in each group.The indexes of cellular immunity,humoral immunity,phagocytic function of monocyte-macrophage and NK cell activity were determined according to the assessment of enhancing immune function in Technical Standards for Testing&Assessment of Health Food after 30 days of continuous oral administration.Results The degree of toes and ankle swelling,antibody forming spleen cells,NK cell activity and the phagocytosis of abdominal macrophages in high-dose group were significantly higher than those in solvent control group(P<0.05).NK cell activity and the phagocytosis of abdominal macrophages in middle-dose group were significantly higher than those in solvent control group(P<0.01).Compared with the solvent control group,there were no significant differences in body weight growth,thymus/body weight ratio,spleen/body weight ratio,level of serum hemolysin,lymphocyte proliferation ability,monocyte-macrophage carbon clearance ability in each dose group(P>0.05).Conclusion The compound yam extract can significantly improve the ability of delayed type hypersensitivity,the number of antibody-producing cells,the ability of macrophages to phagocytosis of chicken red blood cells and the activity of NK in the laboratory mice,which has the function of enhancing immunity.

关 键 词：复方山药提取物 增强免疫力功能 小鼠 

分 类 号：R967[医药卫生—药理学]