4种不同有效成分杀蟑胶饵对德国小蠊的实验室灭效研究  

Laboratory efficacy against Blattella germanica of four cockroach-killing gel baits containing different effective constituents

作　　者：刘曜[1] 刘洪霞[1] 冷培恩[1] 徐劲秋[1] 朱江[1] 范明秋[1] 周毅彬[1] LIU Yao;LIU Hong-xia;LENG Pei-en;XU Jin-qiu;ZHU Jiang;FAN Ming-qiu;ZHOU Yi-bin(Shanghai Municipal Center for Disease Control and Prevention,Shanghai 200336,China)

机构地区：[1]上海市疾病预防控制中心传染病防治所病媒生物防治科,上海200336

出　　处：《中国媒介生物学及控制杂志》2020年第5期559-564,共6页Chinese Journal of Vector Biology and Control

摘　　要：目的研究同一品牌4种不同有效成分杀蟑胶饵对德国小蠊实验室杀灭效果、连锁杀灭效果以及食源对杀灭效果的影响。方法参照国家标准(GB/T 13917.7-2009)对4种杀蟑胶饵进行室内药效测定;使用胶饵毒杀的虫尸喂食进行连锁灭蟑实验;使用胶饵虫尸混合猫粮进行食源干扰连锁灭蟑实验,测定环境中食源对连锁灭蟑效果的影响。使用Excel 2007软件录入数据,采用DPS V 9.01软件计算德国小蠊半数致死时间(LT50)、95%可信区间(95%CI)和毒力回归方程,并进行LT50的显著性分析。采用2种胶饵LT50比率的95%CI作为其检验方法,包含1,则2种药剂LT50之间差异无统计学意义。结果室内药效实验中2.15%吡虫啉、1.00%毒死蜱、0.50%呋虫胺和0.05%氟虫腈胶饵的LT50分别为2.8239(2.5818~3.0790)、0.7455(0.6034~0.8903)、0.7935(0.6309~0.9593)和0.8465(0.4647~1.2280)d,仅吡虫啉组与另外3组的差异有统计学意义(2种胶饵LT50比率的95%CI不含1)。4种胶饵毒杀虫尸连锁灭蟑30 d的死亡率均>98.00%,2.15%吡虫啉、1.00%毒死蜱、0.50%呋虫胺和0.05%氟虫腈胶饵毒杀虫尸组的LT50分别为6.0124(5.2297~6.7545)、2.4590(1.9821~2.9402)、3.6541(3.1501~4.1456)和4.5893(4.0646~5.0888)d,4种胶饵毒杀虫尸LT50两两比较差异均有统计学意义(2种胶饵LT50比率的95%CI不含1)。加入猫粮后连锁灭蟑药效明显减弱,死亡率下降,LT50增大,4种胶饵毒杀虫尸连锁灭蟑有无加入猫粮间差异有统计学意义(2种胶饵LT50比率的95%CI不含1),抑制率分别为120.96%、979.86%、276.83%和207.31%。结论该品牌4种杀蟑胶饵效果良好,1.00%毒死蜱胶饵、0.50%呋虫胺胶饵和0.05%氟虫腈胶饵均能达到快速灭蟑效果。现场灭蟑需要控制食物对胶饵产生的干扰,注意不同药物成分的胶饵交替使用,可达到较好的灭效。Objective To investigate the laboratory efficacy(killing effect,chain-killing effect,and the effect of food source on chain-killing effect)against Blattella germanica of four cockroach-killing gel baits(2.15%imidacloprid gel bait,1.00%chlorpyrifos gel bait,0.50%dinotefuran gel bait,and 0.05%fipronil gel bait)of the same brand.Methods According to the national standard methods(GB/T 13917.7-2009),the laboratory efficacy of the four gel baits was tested;the chain-killing effect was tested by feeding live cockroaches with dead ones killed by the gel baits;the influence of food source on chain-killing effect was tested by feeding live cockroaches with bait-killed cockroaches mixed with cat food.The data were recorded in Excel 2007 software.DPS V 9.01 software was used to calculate the median lethal time(LT50),95%confidence interval(CI),and toxicity regression equation of B.germanica,and the significance of LT50 was analyzed,if 95%CI of LT50 ratio of two baits included 1,there was no significant difference in LT50 between the two baits.Results In the laboratory efficacy test,the LT50 values of 2.15%imidacloprid gel bait,1.00%chlorpyrifos gel bait,0.50%dinotefuran gel bait,and 0.05%fipronil gel bait were 2.8239(2.5818-3.0790)d,0.7455(0.6034-0.8903)d,0.7935(0.6309-0.9593)d,and 0.8465(0.4647-1.2280)d,respectively;only the imidacloprid group showed significant differences from the other three groups(95%CI of LT50 ratio of two baits did not include 1).In the chain efficacy experiment,the mortality rate on day 30 was>98.00%for all the four baits;the LT50 values of the four gel baits were 6.0124(5.2297-6.7545)d,2.4590(1.9821-2.9402)d,3.6541(3.1501-4.1456)d,and 4.5893(4.0646-5.0888)d,respectively;multiple comparisons showed significant differences between any two baits(95%CI of LT50 ratio of two baits did not include 1).After adding cat food for all the four baits,the chain-killing effect was significantly weakened,the mortality rate was decreased,and the LT50 was increased(95%CI of post-pre LT50 ratio did not include 1);the i

关 键 词：杀蟑胶饵 德国小蠊 杀灭效果 连锁灭效 吡虫啉 毒死蜱 呋虫胺 氟虫腈 

分 类 号：S482.3[农业科学—农药学] S481.9[农业科学—植物保护]