耐盐碱溶磷菌Y2R2的分离鉴定及溶磷特性  

Isolation,Identification and Characterization of Salt-alkali-tolerant and Phosphorus-dissolving Bacterium Y2R2

作　　者：杨海霞 刘希旻 潘奕臣 赵香林 黄海东 YANG Hai-xia;LIU Xi-min;PAN Yi-chen;ZHAO Xiang-lin;HUANG Hai-dong(College of Agronomy&Resources and Environment,Tianjin Agricultural University,Tianjin 300384)

机构地区：[1]天津农学院农学与资源环境学院,天津300384

出　　处：《生物技术通报》2020年第10期127-134,共8页Biotechnology Bulletin

基　　金：国家级大学生创新创业训练计划项目(201910061002);国家自然科学基金面上项目(31571790)。

摘　　要：为筛选适应滨海盐碱地环境条件的溶磷菌,从天津滨海新区盐碱土壤中分离得到1株耐盐碱溶磷菌Y2R2,多相分类鉴定结果表明其为盐单胞菌属(Halomonas)的一个新种。菌株Y2R2的生长温度范围为10-45℃,能够耐受pH 14和NaCl浓度15%的生长条件。在低于pH 13和NaCl浓度12%的条件下,菌株Y2R2具有较强的溶磷能力,在pH 8和NaCl浓度9%的无机磷培养基中,发酵48 h溶磷能力最高,达到247.6 mg/L。筛选到的菌株Y2R2为适应滨海盐碱地土壤和气候环境的土著微生物新种,具有较高的耐盐碱和溶磷能力,有望用于耐盐碱溶磷菌剂的开发。The objective of this study is to screen phosphorus-dissolving bacteria with fine environment adaptability to coastal salt-alkali land.Strain Y2R2 with the ability of salt-alkali-tolerant and phosphorus-dissolving was isolated from salt-alkali soil samples collected from Tianjin Binhai New Area.Strain Y2R2 was identified as a novel species of Halomonas according to poly-phase taxonomic characteristics.Strain Y2R2 grew at the temperature 10℃to 45℃,and tolerated the condition of pH 14 and 15%NaCl.Strain Y2R2 showed ability of dissolving phosphorus under pH 7-13 and NaCl content 0-12%.In inorganic phosphorus medium,under the fermentation conditions in which the initial pH was 8,NaCl content was 9%and fermentation time was 48 h,the phosphate-dissolving quantity by strain Y2R2 reached its highest value,247.6 mg/L.Strain Y2R2 screened in this study is a novel species of indigenous microorganisms that adapted to the soil and climate environment of coastal salt-alkali land,and has high salt-alkali tolerance and phosphate-dissolving capacities.The strain is expected to be used as salt-alkali phosphorus-dissolving bacterial agent.

关 键 词：耐盐碱溶磷菌 分类鉴定 盐单胞菌 新种 

分 类 号：S156.4[农业科学—土壤学]