二系杂交小麦亲本光周期、春化和矮秆等位基因的组成和分布  

The composition and distribution of photoperiod,vernalization and dwarf alleles in parents of two-line hybrid wheat

作　　者：陈现朝 张立平[1] 秦志列[1] 廖祥政[1] 孙辉[1] 张风廷[1] 赵昌平[1] CHEN Xianchao;ZHANG Liping;QIN Zhilie;LIAO Xiangzheng;SUN Hui;ZHANG Fengting;ZHAO Changping(Beijing Engineering Research Center For Hybrid Wheat,Beijing Academy of Agriculture and Forestry Sciences/Municipal Key Laboratory of Molecular Genetic of Hybrid Wheat,Beijing 100097,China)

机构地区：[1]北京市农林科学院北京杂交小麦工程技术研究中心/杂交小麦分子遗传北京市重点实验室,北京100097

出　　处：《河北农业大学学报》2020年第5期15-19,45,共6页Journal of Hebei Agricultural University

基　　金：北京市科技计划项目(Z191100004019014);北京市农林科学院青年基金项目(QNJJ201716);北京市农林科学院杰出科学家项目(JKZX201907);北京市农林科学院科技创新能力建设专项(KJCX20200418)。

摘　　要：为了解二系杂交小麦亲本材料光周期、春化和矮秆基因的组成和分布。本研究以245份二系杂交小麦亲本为材料,采用KASP标记对其光周期、春化和矮秆基因型进行鉴定。结果表明:供试材料有3种光周期基因型,即PPd-A1b、PPd-D1b和PPd-B1b,其中PPd-A1b和PPd-D1b在恢复系与不育系中的频率均为100%,PPd-B1b在恢复系与不育系中的频率分别为87.98%和91.67%。供试材料的春化基因型是vrn-A1,在恢复系与不育系中的频率分别为98.71%和100%。恢复系主要含2种矮秆基因型,即Rht-B1a和RhtD1a,其频率分别为98.28%和94.42%,而不育系主要含Rht-B1a和Rht-D1b2种矮秆基因型,其频率分别为66.67%和100%。本研究为杂交小麦适应性遗传改良提供重要的参考;此外,利用标记鉴定并筛选含有优异等位基因遗传材料,有助于提高二系杂交小麦育种效率。To clarify the composition and distribution of photoperiod,vernalization and dwarf alleles in the parent materials of two-line hybrid wheat,a total of 245 parent materials of two-line hybrid wheat were detected by KASP(kompetitive allele specific PCR)markers for photoperiod,vernalization and dwarf genotypes.The results indicated that Ppd-A1 b,Ppd-D1 b and Ppd-B1 b were the three genotypes of photoperiod gene in the tested materials,and the frequency of Ppd-A1 b and Ppd-D1 b were 100%both in restorer and sterile lines,while Ppd-B1 b was 87.98%and 91.67%in restorer and sterile lines,respectively.The genotype of vernalization gene was vrn-A1 in the tested materials,the frequency of which was 98.71%and 100%in restorer and sterile lines,respectively.The dwarf genes Rht-B1 a and Rht-D1 a were present in the restorer lines with the frequency of 98.28%and 94.42%,respectively.RhtB1 a and Rht-D1 b were observed in the sterile lines with the frequency of 66.67%and 100%,respectively.This study has significant value for further development of adaptive genetic improvement of hybrid wheat.In addition,KASP assays could greatly improve speed and efficiency of the two-line hybrid wheat breeding programs by identification and screening of genetic materials with more valuable allelic genes.

关 键 词：二系杂交小麦亲本 光周期 春化 矮秆 基因等位变异 

分 类 号：S512.1[农业科学—作物学]