玉米BPC家族基因的生物信息学与表达规律分析  

Analysis of bioinformatics and expression regularity of maize BPC family genes

作　　者：周帆[1] 米淑玲 张晓燕[1] 藏金萍[1] 张康 邢继红[1] 董金皋[1] ZHOU Fan;MI Shuling;ZHANG Xiaoyan;ZANG Jinping;ZHANG Kang;XING Jihong;DONG Jingao(Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology/Mycotoxin and Molecular Plant Pathology Laboratory of Hebei Agricultural University,Baoding 071000,China;Agriculture Bureau of Yunhe District,Cangzhou 061000,China)

机构地区：[1]河北省植物生理与分子病理学重点实验室/河北农业大学真菌毒素与植物分子病理学实验室,河北保定071000 [2]河北省沧州市运河区农业局,河北沧州061000

出　　处：《河北农业大学学报》2020年第5期27-33,共7页Journal of Hebei Agricultural University

基　　金：粮食丰产增效科技创新专项(2016YFD0300704);国家自然科学基金项目(31901864);河北省自然科学基金项目(C2019204141)。

摘　　要：BASIC PENTACYSTEINE(BPC)基因家族是植物特异性的转录因子家族,在基因转录调控过程中发挥重要的作用。为探究玉米BPC家族基因的功能及其调控机制,本研究利用生物信息学技术,对玉米BPC家族基因进行系统进化分析、基因结构分析、保守结构域分析、组织表达特性分析以及在玉米抵抗非生物胁迫过程中的表达规律分析。结果发现,玉米的4个BPC家族基因均含有保守的GAGA结合结构域,具有明显的组织表达特性,在热、冷、盐、UV和干旱胁迫下呈现不同的表达规律。利用Real-time PCR技术,检测玉米BPC家族基因在不同激素处理后表达水平,发现4个BPC家族基因在水杨酸、茉莉酸、乙烯利处理后的表达水平均发生明显的变化。结果表明BPC家族基因在玉米生长发育以及抵抗非生物胁迫过程中发挥重要作用,为阐明玉米BPC家族基因的功能及其调控机制奠定了基础。BASIC PENTACYSTEINE(BPC)gene family is a plant-specific family of transcription factors that plays an important role in the regulation of gene transcription.In order to explore the function and regulation mechanism of maize BPC family genes,this study used bioinformatics technology to conduct systematic evolutionary analysis,gene structure analysis,conserved domain analysis,tissue expression characteristics analysis and analysis of expression law in resistance to non-biological genes in maize BPC family genes.It was found that the four BPC family genes of maize all contained conserved GAGA binding domains,which had obvious tissue expression characteristics,and showed different expression rules under heat,cold,salt,UV and drought stress.Real-time PCR technology was used to detect the expression levels of maize BPC family genes after treatment with different hormones,and it was found that the expression levels of the four BPC family genes after salicylic acid,jasmonic acid,and ethephon treatment changed significantly.The results show that the BPC family genes play an important role in the growth and development of corn and resistance to abiotic stress,which lays a foundation for elucidating the function and regulatory mechanism of maize BPC family genes.

关 键 词：玉米 BPC 生物信息学分析 Real time-PCR 

分 类 号：S432.1[农业科学—植物病理学]