BtCry1Ac原毒素对杨扇舟蛾幼虫的毒力测定及幼虫对Bt毒素的细胞响应  

Virulence of BtCry1Ac protoxin to the larvae of Clostera anachoreta and the cellular response of larvae to Bt toxin

作　　者：刘洁[1] 高宝嘉[1] LIU Jie;GAO Baojia(College of Forestry,Hebei Agricultural University,Baoding 071000,China)

机构地区：[1]河北农业大学林学院,河北保定071000

出　　处：《河北农业大学学报》2020年第5期68-72,共5页Journal of Hebei Agricultural University

摘　　要：为探究BtCry1Ac毒蛋白对杨扇舟蛾(Clostera anachoreta)中肠的作用及其机制,本研究通过BtCry1Ac原毒素对杨扇舟蛾幼虫进行胁迫,测定毒素对幼虫的LC50,并观察杨扇舟蛾中肠细胞的电镜结构。结果表明,幼虫取食Bt毒素2 h后,中肠微绒毛即出现断裂脱落情况;内质网肿胀排列不规则;线粒体变形;细胞核染色质发生固缩,随着胁迫时间的延长,中肠结构发生严重病变。研究还发现,其中肠细胞内观察到自噬体和吞噬体等结构,说明Bt的胁迫可能引起中肠细胞的自噬反应。In order to explore the effect of BtCry1 Ac toxin on the midgut of Clostera anachoreta and its mechanism,this study used BtCry1 Ac protoxin to determine the LC50 of the toxin on the larvae of C.anachoreta,and to observe the electron microscope structure of the midgut cells of C.anachoreta.The results showed that after feeding Bt toxin for 2 h,the microvilli of the midgut were broken off,the endoplasmic reticulum was swollen and arranged irregularly,the mitochondria were deformed,and the chromatin of the nucleus was condensed.With the extension of stress time,the lesions of those structures were aggravated.It was also found that autophagy and phagocytic structures were observed in intestinal cells,indicating that Bt stress might induce autophagy in midgut cells.

关 键 词：杨扇舟蛾 BtCry1Ac 细胞结构 自噬反应 

分 类 号：S718.5[农业科学—林学]