黄芪多糖纳米粒的构建及特征  

Construction and Characteristics of Astragalus Polysaccharide Nanoparticles

作　　者：许小英[1] 周存敏[1] 谭榜云[1] 陈琳[1] 朱海平[1] XU Xiaoying;ZHOU Cunmin;TANG Bangyun;CHEN Lin;ZHU Haiping(Department of Laboratory Medicine,the First Hospital of Lanzhou University,Lanzhou 730000)

机构地区：[1]兰州大学第一医院检验科,兰州730000

出　　处：《药学与临床研究》2020年第5期333-337,共5页Pharmaceutical and Clinical Research

基　　金：甘肃省中医药管理局科研课题(GZK-2016087);兰州大学第一医院院内基金资助项目(ldyyyn2017-05)。

摘　　要：目的:借助纳米技术制备成APS纳米粒。方法:根据离子交联原理制备APS纳米粒。通过红外吸收光谱分析、粒径、Zeta电位,表征其物理特性,并将该纳米粒与大鼠心肌细胞共培养,评价细胞相容性。结果:该药物的红外吸收光谱在618.3 cm^-1处出现特征吸收峰,平均粒径为112.4 nm,Zeta电位为(41.0±0.21)mV,与H9c2细胞共培养后细胞活力无显著性变化(P=0.557)。结论:制成APS纳米粒,此纳米粒粒径分布均匀,性质稳定,具有良好的细胞相容性。Objective:The astragalus polysaccharide nanoparticles(APS-CS NPs)were prepared by the nanotechnology for the application of traditional Chinese medicine.Methods:APS-CS NPs were prepared using the ionic gelation technique.Then fourier transform infrared(FTIR)spectra,sizes and zeta potentials were analyzed to evaluate their physical characteristics.Finally,they were co-cultured with cardiomyocytes,and their cell compatibility were evaluated.Results:FTIR spectra of the APS-CS NPs showed a characteristic absorption peak at the frequency of 618.3 cm-1.The average particle size was 112.4 nm and the zeta potential was 41.0±0.21.There was no significant change in cell viability after co-cultivation of the nanomedicine with H9c2 cells(P=0.557).Conclusion:APS-CS NPs with uniform particle size,regular morphology and good biocompatibility has been successfully prepared.

关 键 词：黄芪多糖 纳米粒 物理特征 

分 类 号：R943[医药卫生—药剂学] TQ460.6[医药卫生—药学]