一例异育银鲫病源细菌的分离、鉴定及药敏试验  

Isolation,identification and drug sensitivity test of pathogenic bacteria from a case of Carassius auratus gibelio

作　　者：张荧荧 刘张淮 朱春艳 王晓英 管杰 王宇哲 王家军 Zhang Yingying;Liu Zhanghuai;Zhu Chunyan;Wang Xiaoying;Guan Jie;Wang Yuzhe;Wang Jiajun(Center for prevention and control of aquatic animal diseases,Baoying County,Jiangsu Province,225800)

机构地区：[1]宝应县水生动物疫病与预防控制中心,江苏宝应225800

出　　处：《水产养殖》2020年第10期1-4,共4页Journal of Aquaculture

基　　金：江苏省水生动物病害测报项目。

摘　　要：该试验从异育银鲫(Carassius auratus gibelio)病灶部位分离出3种病菌,使用细菌学方法分析它们的形态特征,进行16 S rRNA基因扩增和测序,并结合生理生化特性鉴定细菌种类。同时对3种病菌进行药敏试验。结果显示3种病菌分别为栖稻黄色单胞菌(Pseudomonas oryzihabitans)、嗜水气单胞菌(Aeromonas hydrophila)、恶臭假单胞菌(Pseudomonas putida)。栖稻黄色单胞菌对恩诺沙星、硫酸新霉素、盐酸多西环素和磺胺甲恶唑+甲氧苄啶高度敏感。嗜水气单胞菌对恩诺沙星、硫酸新霉素、甲砜霉素、氟苯尼考和盐酸多西环素五种抗生素高度敏感。恶臭假单胞菌对恩诺沙星、硫酸新霉素、盐酸多西环素高度敏感。In this study,three pathogenic bacterium,which were identified by morphological analysis and 16S rRNA sequencing technique,were isolated from the lesion of Carassius auratus gibelio.Furthermore,drug sensitivity tests were conducted on these pathogenic bacterium.The results showed that the three pathogenic bacterium were Aeromonas hydrophila,Pseudomonas oryzihabitans and P.putida.A.hydrophila was highly sensitive to enrofloxacin,neomycin sulfate,thiamphenicol and florfenicol,as well as doxycycline hydrochloride.P.oryzihabitans was significantly sensitive to enrofloxacin,neomycin sulfate,doxycycline hydrochloride and sulfamethoxazole+trimethoprim.P.putida was highly sensitive to enrofloxacin,neomycin sulfate,and doxycycline hydrochloride.

关 键 词：异育银鲫(Carassius auratus gibelio) 细菌分离鉴定 药敏试验 

分 类 号：S941[农业科学—水产养殖]