基于SDF-1/CXCR4信号轴研究肺复方对肺癌A549细胞侵袭能力的影响  

Effects of Feifufang on Lung Cancer A549 Cells Invasion Based on SDF-1/CXCR4 Axis

作　　者：谭小宁[1] 朱克俭[1] 蒋益兰[1] 罗吉[1] 罗燕[1] 吕元[1] 马思静 李勇敏[1] TAN Xiaoning;ZHU Kejian;JIANG Yilan;LUO Ji;LUO Yan;LYU Yuan;MA Sijing;LI Yongmin(The Affiliated Hospital of Hunan Academy of Chinese Medicine,Hunan Key Laboratory of Chinese Medicine Oncology,Innovative Platform of Anti-tumor Chinese Drugs,Changsha 410006,China)

机构地区：[1]湖南省中医药研究院附属医院,中医肿瘤学湖南省重点实验室,抗肿瘤中药创新平台,湖南长沙410006

出　　处：《中国中医药信息杂志》2020年第9期58-62,共5页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81503452、81803787);湖南省中医药研究院重点项目(201804)。

摘　　要：目的观察肺复方对肺癌A549细胞侵袭能力的影响,从SDF-1/CXCR4生物轴调控角度探讨其作用机制。方法实验分为空白血清组(正常血清培养基)和肺复方低、中、高剂量组(分别加入5%、10%、15%肺复方药物血清培养基),各组加入基质衍生因子1(SDF-1)处理48 h,Transwell检测A549细胞侵袭能力,Western blot检测A549细胞SDF-1特异受体CXCR4、磷脂酰肌醇3-激酶(PI3K)、蛋白激酶B(Akt)、p-Akt、E-钙黏蛋白(E-cadherin)、波形蛋白(Vimentin)蛋白表达,RT-PCR检测A549细胞E-cadherin、Vimentin和CXCR4基因表达。结果与空白血清组比较,肺复方低、中、高剂量组A549细胞体外侵袭能力明显下降(P<0.01);与空白血清组比较,肺复方低、中、高剂量组A549细胞CXCR4、PI3K、Akt、p-Akt和Vimentin蛋白表达均有一定程度下降,E-cadherin蛋白表达上调,差异均有统计学意义(P<0.05,P<0.01),肺复方低、中、高剂量组A549细胞CXCR4、Vimentin mRNA表达下调,肺复方中、高剂量组A549细胞E-cadherin mRNA表达上调,差异均有统计学意义(P<0.05,P<0.01)。结论肺复方能抑制A549细胞侵袭能力、上皮间质转化进程,其作用机制可能与SDF-1/CXCR4生物轴调控PI3K-Akt通路有关。Objective To observe the effects of Feifufang on the invasion ability of lung cancer A549 cells;To explore the mechanism of action from the perspective of SDF-1/CXCR4 biological axis regulation.Methods The experiment was divided into blank serum group(normal serum medium)and Feifufang low-,medium-,and high-dosage groups(adding 5%,10%and 15%Feifufang serum medium respectively).Each group was treated with matrix derived factor 1(SDF-1)for 48 h.Transwell was used to detect the invasion ability of A549 cells.Western Blot was used to detect the expressions of SDF-1 specific receptor CXCR4,phosphatidylinositol 3-kinase(PI3K),protein kinase B(Akt),p-Akt,E-cadherin and Vimentin in each group.The gene expression levels of E-cadherin,Vimentin and CXCR4 were detected by RT-PCR.Results Compared with the blank serum group,the invasion ability of A549 cells in Feifufang low-,medium-and high-dosage groups was significantly reduced(P<0.01).Compared with the blank serum group,the protein expressions of CXCR4,PI3K,Akt,p-Akt and Vimentin of A549 cells in the Feifufang low-,medium-and high-dosage groups all decreased to a certain extent,and the protein expression of E-cadherin was up-regulated,with statistical significance(P<0.05,P<0.01);the mRNA expressions of CXCR4 and Vimentin in A549 cells were down-regulated in Feifufang low-,medium-,and high-dosage groups,while the expression of E-cadherin mRNA in A549 cells in Feifufang medium-and high-dosage groups was up-regulated,with statistical significance(P<0.05,P<0.01).Conclusion Feifufang can inhibit the invasion ability and the epithelial-mesenchymal transition process of A549 cells,and the mechanism may be related to the biological axis regulation of SDF-1/CXCR4 on PI3K/Akt pathway.

关 键 词：肺癌 肺复方 SDF-1/CXCR4生物轴 上皮间质转化 A549细胞 

分 类 号：R285.5[医药卫生—中药学]