胡敏酸和富里酸对土壤中DnBP降解及微生物数量的影响规律  

Effects of Humic Acid and Fulvic Acid on DnBP Degradation Rate and Microbial Quantity in Soil

作　　者：李玉双 刘厶瑶 赵晓旭 宋雪英 侯永侠 魏建兵 徐硕 LI Yushuang;LIU Siyao;ZHAO Xiaoxu;SONG Xueying;HOU Yongxia;WEI Jianbing;XU Shuo(Key Laboratory of Regional Pollution Environment and Ecological Rehabilitation, Ministry of Education, School of Environment, Shenyang University, Shenyang 110044, China;School of Environmental and Biological Engineering, Putian University, Putian 351100, China)

机构地区：[1]沈阳大学环境学院,辽宁沈阳110044 [2]莆田学院环境与生物工程学院,福建莆田351100

出　　处：《沈阳大学学报（自然科学版）》2020年第5期381-386,共6页Journal of Shenyang University：Natural Science

基　　金：国家自然科学基金青年科学基金资助项目(21307084);国家自然科学基金面上项目(41771200,41171399);辽宁省自然科学基金资助项目(2019-ZD-0550);生态环境及其信息图谱福建省高等学校重点实验室(莆田学院)开放经费资助课题(ST19003).

摘　　要：采用土壤培养实验结合气相色谱技术和平板菌落计数法,研究了胡敏酸(HA)和富里酸(FA)对土壤中邻苯二甲酸正二丁酯(DnBP)降解的影响规律及其与土壤微生物数量变化的关系.结果表明,HA和FA的添加促进了土壤微生物对DnBP的降解,DnBP的降解率随HA和FA添加量的增加呈先升高后降低的趋势.HA和FA的最适添加量分别为80和40 mg·g^-1,该用量处理5 d时DnBP的降解率分别达68.6%和74.5%,为对照处理的1.4和1.5倍.HA和FA的添加促进了土壤细菌和真菌的增殖,微生物数量随HA和FA添加量的增加也呈先增加后减少趋势.HA处理组细菌数量和FA处理组的细菌和真菌数量均在添加量为40 mg·g^-1时出现最大值,而HA处理组真菌的数量则在添加量为80 mg·g^-1时呈现最大值.相关分析结果表明HA和FA的添加促进了土壤细菌和真菌的繁殖,进而促进了土壤微生物对DnBP的降解.The effects of HA and FA on the degradation of n-dibutyl phthalate(DnBP)in soil and its relationship with changes in soil microorganisms were studied using soil culture experiments combined with gas chromatography and plate colony counting.The results showed that the addition of HA and FA promoted the degradation of DnBP by soil microorganisms,and the degradation rate of DnBP showed a trend of first increase and then decrease with the increase of the amount of HA and FA.The optimal addition amounts of HA and FA were 80 mg·g^-1 and 40 mg·g^-1,respectively.The degradation rates of DnBP reached 68.6%and 74.5%when treated for 5 days,which were 1.4 and 1.5 times of the control treatment,respectively.The addition of HA and FA promoted the proliferation of soil bacteria and fungi.The number of microorganisms also increased and then decreased with the addition of HA and FA.The maximum number of bacteria in the HA treatment group and the number of bacteria and fungi in the FA treatment group appeared when the concentration was 40 mg·g^-1,while the maximum number of fungi in the HA treatment group showed a maximum when the concentration was 80 mg·g^-1.The related analysis results show that the addition of HA and FA promotes the reproduction of soil bacteria and fungi,and then promotes the degradation of DnBP by soil microorganisms.

关 键 词：土壤 胡敏酸 富里酸 DNBP 微生物数量 

分 类 号：X53[环境科学与工程—环境工程]