吴茱萸碱对胶质瘤SHG-44细胞凋亡的促进作用及其机制  

Evodiamine Promotes Apoptosis of Glioma SHG-44 Cells and Its Mechanism

作　　者：刘璐[1] 王雪梅 王岩[1] 席红梅 郭建多 黄求进[1] LIU Lu;WANG Xuemei;WANG Yan;XI Hongmei;GUO Jianduo;HUANG Qiujin(Stroke Epilepsy Wards,First Affiliated Hospital of Harbin Medical University,Harbin 150001,China)

机构地区：[1]哈尔滨医科大学附属第一医院脑卒中癫痫病房,哈尔滨150001

出　　处：《中国医学科学院学报》2020年第5期591-595,共5页Acta Academiae Medicinae Sinicae

摘　　要：目的研究吴茱萸碱对人胶质瘤SHG-44细胞凋亡的促进用及其机制。方法将体外培养的胶质瘤SHG-44细胞分为对照组、吴茱萸碱组(根据吴茱萸碱浓度不同分为3个亚组),CCK-8法观察细胞活性,流式细胞仪测定细胞凋亡率,Hoechst 33258核染色法观察细胞核凋亡,透射电镜观察细胞形态学的变化,Western blot法检测胶质瘤SHG-44细胞内Cleaved Caspase-3及Cleaved Caspase-9的蛋白表达。结果吴茱萸碱对胶质瘤SHG-44细胞增殖有抑制作用。流式细胞仪及Hoechst 33258核染色法的检测结果表明,随着吴茱萸碱作用浓度的递增,胶质瘤SHG-44细胞凋亡率呈剂量依赖性递增;吴茱萸碱作用后可使Cleaved Caspase-3及Cleaved Caspase-9蛋白的表达升高。结论吴茱萸碱通过改变Cleaved Caspase-3及Cleaved Caspase-9蛋白的表达,抑制胶质瘤SHG-44细胞增殖并促进其凋亡。Objective To explore the role of evodiamine in promoting the apoptosis of glioma SHG-44 cells and its mechanism.Methods The in vitro cultured glioma SHG-44 cells were divided into control group and evodiamine group(which was further divided into three subgroups according to the glycoside concentrations).Cell viability was determined by CCK-8 method,cells apoptosis rate by flow cytometry,and nucleus apoptosis by Hoechst 33258 nuclear staining.Cell morphological changes were observed by transmission electron microscope.Protein expressions of Cleaved Caspase-3 and Cleaved Caspase-9 were detected by Western blot analysis.Results Evodiamine significantly inhibited the proliferation of glioma SHG-44 cells.The apoptosis rate of Glioma cells increased in a dose-dependent manner as the evodiamine concentration increased.Evodiamine promoted the expressions of cleaved Caspase-3 and cleaved Caspase-9.Conclusion Evodiamine inhibits glioma cell proliferation by changing the expressions of cleaved Caspase-3 and cleaved Caspase-9.

关 键 词：吴茱萸碱 胶质瘤SHG-44细胞 增殖 凋亡 

分 类 号：R34[医药卫生—基础医学]