基于核质遗传原理建立多重PCR检测方法鉴定阿胶中马、驴源性成分及皮张种源  

A multiplex PCR method based on nuclear and cytoplasmicinheritance to identify the horse and donkey-derivedcomponents of Asini Colla Corii and the hide

作　　者：谌阳 王文君[1] 付明 徐国强 周翔[1] 刘榜[1] Yang Shen;Wenjun Wang;Ming Fu;Guoqiang Xu;Xiang Zhou;Bang Liu(Key Laboratory of Agricultural Animal Genetics,Breeding and Reproduction of Ministry of Education,College of Animal Science and Technology,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]华中农业大学动物科学技术学院,农业动物遗传育种与繁殖教育部重点实验室,武汉430070

出　　处：《遗传》2020年第10期1028-1035,共8页Hereditas（Beijing)

基　　金：国家科技重大专项(编号:2018ZX08012-001)资助。

摘　　要：阿胶(Asini Colla Corii)及其原料皮张源性成分的鉴定是对阿胶真实性的一种保障,阿胶生产企业和市场监管部门急需马、驴、骡皮张以及阿胶中源性成分鉴别的有效检测方法。本研究基于马、驴核基因组和线粒体基因组筛选物种特异性DNA序列作为检测靶标,设计马、驴特异性引物,建立了鉴别马、驴、骡皮张以及鉴定阿胶中马和驴源性成分的多重PCR方法。结果显示,本文所建立的方法可用于阿胶源性成分及皮张种源的鉴别,其特异性强,只在目标物种中扩增出目的条带,而非目标物种中没有任何扩增产物,而且灵敏度能达到0.2 ng,可为阿胶生产企业和市场监管部门提供技术支撑。To identify the original components of Asini Colla Corii and its raw material hides provides a guarantee for authenticity of Asini Colla Corii.It is urgent for Asini Colla Corii production enterprises and market supervision departments to develop effective identification methods of Asini Colla Corii and hides derived from horses,donkeys,mules and hinnies.This study screened species-specific DNA sequences of nuclear and mitochondrial genomes as detection targets,designed horse and donkey specific primers and established multiple PCR identification methods for identifying the animal hides(including the horse,donkey,mule and hinny)and Asini Colla Corii containing horse-derived and donkey-derived components.Our method can identify the horse,donkey,mule and hinny hides and horse,donkey-derived components of Asini Colla Corii with high species specificity(no crossed amplification was observed).The limit of detection was 0.2 ng DNA. The method developed in this study provides technical support for Asini Colla Corii production enterprises andmarket supervision departments.

关 键 词：阿胶 核基因 线粒体基因 多重PCR 马、驴源性成分 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]