GC-FID内标法测定复方丹参制剂醇沉上清液乙醇含量  

Determination of ethanol content in supernatant of compound salvia miltiorrhiza preparation by GC-FID internal standard method

作　　者：赵波 熊皓舒 赵万顺 章顺楠 ZHAO Bo;XIONG Hao-shu;ZHAO Wan-shun;ZHANG Shun-nan(School of Traditional Chinese Pharmacy,China Pharmaceutical University,Nanjing 211198,China;National&Local United Engineering Laboratory of Advanced Manufacturing Technology of traditional Chinese Medicine,Tasly Pharmaceutical Group Co.Ltd.,Tianjin 300400,China)

机构地区：[1]中国药科大学中药学院,江苏南京211198 [2]中药先进制造技术国家地方联合工程实验室,天士力医药集团股份有限公司,天津300400

出　　处：《化学工程师》2020年第7期21-23,57,共4页Chemical Engineer

基　　金：2018年度天津市智能制造专项资金项目(201810135)。

摘　　要：目的建立GC-FID内标法,快速、准确测定复方丹参制剂醇沉上清液乙醇含量。方法以正丙醇为内标物,采用AgiLent DB-624(30m×0.53mm×3μm)色谱柱,氢火焰离子化检测器检测。结果该方法在乙醇体积分数为0.00999%~0.069930%范围内呈现良好的线性关系(R2=0.9998);加标回收率为97.39%~102.54%,RSD为0.53%~1.56%。结论方法简便快捷、准确可靠,可用于复方丹参醇沉上清液乙醇含量的测定。OBJECTIVE To establish a GC-FID internal standard method to quickly and accurately determine the ethanol content in supernatant of compound Salvia miltiorrhiza preparation.METHODS Using n-propanol as internal standard,the determination was carried out by an AgiLent DB-624(30m×0.53mm×3μm)chromatographic column with a hydrogen flame ionization detector.RESULTS The method showed a good linear relationship(R2=0.9998)in the range of 0.00999%~0.069930%volume fraction of ethanol,and the recovery rate of addition standard was 97.39%~102.54%,RSD was 0.53%~1.56%.CONCLUSION The method is fast,accurate and reliable,and can be used for the determination of ethanol content in the supernatant of compound Salvia miltiorrhiza.

关 键 词：气相 内标法 上清液 乙醇 正丙醇 

分 类 号：R284.2[医药卫生—中药学]