多囊卵巢综合征患者子宫内膜的蛋白质组学研究  

Proteomics study of endometrium in patients with polycystic ovarian syndrome

作　　者：朱宇 王磊 赵红翠[3] 甄秀梅[3] 马旭 高华方 ZHU Yu;WANG Lei;ZHAO Hongcui;ZHEN Xiumei;MA Xu;GAO Huafang(Graduate School of Peking Union Medical College,Beijing,100730;National Research Institute for Family Planning;Peking University Third Hospital)

机构地区：[1]北京协和医学院研究生院,100730 [2]国家卫生健康委科学技术研究所 [3]北京大学第三医院

出　　处：《中国计划生育学杂志》2020年第10期1526-1528,1721,共4页Chinese Journal of Family Planning

基　　金：国家重点研发项目(2016YFC1000302)。

摘　　要：目的:应用液相色谱串联质谱(LC-MS/MS)技术对多囊卵巢综合征(PCOS)患者子宫内膜组织进行蛋白质组学研究,寻找差异表达蛋白及其相关生物通路。方法:将符合入组条件的不孕妇女子宫内膜活检组织样本分为PCOS组(n=15)和非PCOS组(n=12),LC-MS/MS技术检测,对数据进行统计学和生物信息学分析。结果:共鉴定出7285种蛋白,其中6920种蛋白在两组样本中均表达,PCOS组上调表达蛋白290种(FC≥2.0,P<0.05),下调表达蛋白141种(FC≤0.5,P<0.05)。对蛋白进行京都基因与基因组百科全书(KEGG)富集分析,结果差异蛋白在色氨酸代谢,酪氨酸代谢和苯丙氨酸代谢通路中富集明显,其中色氨酸代谢通路富集最显著,涉及的差异蛋白为犬尿氨酸3单加氧酶、醛氧化酶1、胺氧化酶、HCG23341蛋白、胺氧化酶(含黄素)A、胺氧化酶(含黄素)B、2氧戊二酸脱氢酶。结论:PCOS患者子宫内膜可能存在氨基酸代谢异常,产生免疫稳态失衡、细胞增殖功能和供能改变,这种改变可能与其致病机制有关。Objective:To identify the proteomics of endometrium in patients with polycystic ovarian syndrome(PCOS)by liquid chromatography tandem mass spectrometry(LC-MS/MS)technology,and to find proteins expressed differentially and their associated biological pathways.Methods:Endometrial biopsy tissue samples were divided into PCOS group(n=15)and non PCOS group(n=12).After these tissues were processed,the LC-MS/MS technology was used to detect the solution samples,then the offline data was statistically and bioinformatically analyzed.Results:A total of 7285 proteins were identified,of which 6920 proteins were expressed in both groups.In the PCOS group,the expression of 290 proteins had up regulated(FC≥2.0,P<0.05),and the expression of 141 proteins had down regulated(FC≤0.5,P<0.05).KEGG enrichment analysis of these proteins had showed that differentially expressed proteins on the tryptophan metabolic pathway,the tyrosine metabolic pathway,and the phenylalanine metabolic pathway were significant enriched,of which tryptophan metabolism was the most significant.The differential proteins involved Kynurenine 3 monooxygenase,AOX1,Amine oxidase,HCG23341,Amine oxidase(flavin containing)A,Amine oxidase(flavin containing)B,2 oxoglutarate dehydrogenase.Conclusion:There is abnormal amino acid metabolism in protein expression levels in the endometrium of the patients with PCOS,which may lead to immune homeostasis,the changes of proliferative function and energy supply of cell,and may be associated with pathogenic mechanism of PCOS.

关 键 词：多囊卵巢综合征 液相色谱串联质谱 子宫内膜 蛋白质组学 氨基酸代谢 

分 类 号：R711.75[医药卫生—妇产科学]