HPLC法同时测定前列消胶囊中9种成分  

Simultaneous determination of nine constituents in Qianliexiao Capsules by HPLC

作　　者：焦健 董伟 赵善冬 任维鑫 张超[2] JIAO Jian;DONG Wei;ZHAO Shan-dong;REN Wei-xin;ZHANG Chao(Jinan Municipal People’s Hospital,Jinan 271199,China;College of Traditional Chinese Medicine,Shandong University of Traditional Chinese Medicine,Jinan 250355,China)

机构地区：[1]济南市人民医院,山东济南271199 [2]山东中医药大学中药学院,山东济南250355

出　　处：《中成药》2020年第10期2566-2569,共4页Chinese Traditional Patent Medicine

摘　　要：目的建立HPLC法同时测定前列消胶囊(虎杖、土茯苓、泽泻等)中虎杖苷、落新妇苷、花旗松素、黄杞苷、泽泻醇F、泽泻醇A、24-乙酰泽泻醇A、泽泻醇G、23-乙酰泽泻醇B的含有量。方法该药物60%乙醇提取液的分析采用Kromasil C18色谱柱(250 mm×4.6 mm,5μm);流动相乙腈-0.1%冰醋酸,梯度洗脱;体积流量1.0 mL/min;柱温30℃;检测波长208、291 nm。结果9种成分在各自范围内线性关系良好(r≥0.9992),平均加样回收率96.94%~100.09%,RSD 0.77%~1.42%。结论该方法简便准确,重复性好,可用于前列消胶囊的质量控制。AIM To establish an HPLC method for the simultaneous content determination of polydatin,astilbin,taxifolin,engeletin,alisol F,alisol A,24-acetate alisol A,alisol G and 23-acetate alisol B in Qianliexiao Capsules(Polygoni cuspidati Rhizoma et Radix,Smilacis glabrae Rhizoma,Alismatis Rhizoma,etc.).METHODS The analysis of 60% ethanol extract of this drug was performed on a 30℃ thermostatic Kromasil C18 column(250 mm×4.6 mm,5μm),with the mobile phase comprising of acetonitrile-0.1% glacial acetic acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 208,291 nm.RESULTS Nine constituents showed good linear relationships within their own ranges(r≥0.9992),whose average recoveries were 96.94%-100.09% with the RSDs of 0.77%-1.42%.CONCLUSION This simple,accurate and reproducible method can be used for the quality control of Qianliexiao Capsules.

关 键 词：前列消胶囊 化学成分 HPLC 

分 类 号：R927.2[医药卫生—药学]