不同储藏年限地黄化学成分分析  

Analysis of chemical constituents from Rehmannia glutinosa with different storage years

作　　者：宋琳琳 薛淑娟[1] 王利丽[1] 张飞[1] 陈随清[1] SONG Lin-lin;XUE Shu-juan;WANG Li-li;ZHANG Fei;CHEN Sui-qing(School of Pharmacy,Henan University of Traditional Chinese Medicine,Zhengzhou 450046,China)

机构地区：[1]河南中医药大学药学院,河南郑州450046

出　　处：《中成药》2020年第10期2661-2669,共9页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金(81973477)。

摘　　要：目的分析不同储藏年限地黄的化学成分。方法对地黄中氨基酸含有量进行分析,硫酸-苯酚法测定地黄总多糖含有量,HPLC法测定毛蕊花糖苷、环烯醚萜苷类、单糖、寡糖含有量。结果随着储存时间增加,总氨基酸含有量下降,其中碱性氨基酸赖氨酸和精氨酸幅度较大;棉籽糖、水苏糖、总多糖含有量逐年降低,果糖、葡萄糖、蔗糖、甘露三糖含有量呈现先增后减的趋势,蜜二糖含有量基本平稳;单糖中半乳糖、甘露糖、阿拉伯糖随着储存时间的增加含有量逐年增加,环烯醚萜苷类成分梓醇、地黄苷D、地黄苷A、益母草苷随着储藏年限的延长,成分逐渐降低;毛蕊花糖苷含有量也是下降的。结论随着地黄储藏年限的增加,断面越来越黑,大多数成分含有量在储藏3年后趋于平稳。AIM To analyze the chemical constituents from Rehmannia glutinosa Libosch with different storage years.METHODS The content of amino acids in R.glutinosa was analyzed,and the content of total polysaccharides was determined by sulfuric acid-phenol method.HPLC was established to determine the content of verbascoside,cycloene terpenoids,monosaccharides and oligosaccharides.RESULTS With increasing the storage years,the total amino acid content decreased,among which the basic amino acids lysine and arginine had a larger decline,and the contents of raffinose,stachyose,and total polysaccharides decreased year by year.The content of fructose,glucose,sucrose and manninotriose increased first then decreased,and the content of melibiose was basically stable.With increasing the storage years,the contents of galactose,mannose and arabinose in monosaccharides increased year by year,and the contents of cyclic allene terpenoids catalpol,digoxigenin D,digoxigenin A,and motherwort glycosides,as well as verbascoside decreased dgradually.CONCLUSION With the storage years increasing of R.glutinosa,the cross section becomes dark increasingly,and the contents of most chemical constituents have stabilized after three storage years.

关 键 词：地黄 化学成分 储藏年限 硫酸-苯酚法 HPLC 

分 类 号：R284.1[医药卫生—中药学]