基于茶树油/盐酸奥替尼啶的纳米胶囊的制备及抗菌性能  

Preparation and Antibacterial Properties of Nanocapsules Based on Tea Tree Oil/Octenidine·2HCl

作　　者：邢幸 康宁 曾天 徐云龙[1] XING Xing;KANG Ning;ZENG Tian;XU Yunlong(School of Materials Science and Engineering,East China University of Science and Technology,Shanghai 200237,China;Shanghai Velatech Co.,Shanghai 200237,China)

机构地区：[1]华东理工大学材料科学与工程学院,上海200237 [2]上海微纳科技有限公司,上海200237

出　　处：《华东理工大学学报（自然科学版）》2020年第5期621-630,共10页Journal of East China University of Science and Technology

基　　金：上海市纳米技术专项基金(11nm0500900);上海市学科带头人(B502);上海市重点实验室项目(08DZ2230500)。

摘　　要：以双吡啶胺类广谱抗菌药盐酸奥替尼啶(OCT,Octenidine·2HCl)为乳化剂,正硅酸乙酯(TEOS)为硅源,用微乳液法成功制备出包裹茶树油(TTO)的纳米二氧化硅胶囊SINCOCT/TTO。随后仅将乳化剂更换为十六烷基三甲基氯化铵(CTAC),以同样的方法和配比制备包裹茶树油的纳米二氧化硅胶囊SINC-TTO。通过扫描电镜、透射电镜、粒度分析、红外光谱、热重分析、氮气吸附-脱附等表征方法对SINC-OCT/TTO和SINC-TTO这两种纳米胶囊(SINCs)进行形貌和结构表征,并进行抗菌性能和释药性能测试。结果表明:两种纳米胶囊呈规整的空心球形结构并且具有良好的药物缓释性能。纳米胶囊的结构受乳化剂的种类影响,SINC-OCT/TTO的流体力学直径、比表面积、包药量均大于SINC-TTO。联合药敏实验分析结果表明,TTO与OCT联用可以对金黄色葡萄球菌起到协同抗菌作用;对大肠杆菌起到相加抗菌作用。纳米胶囊的抗菌实验结果证实SINC-OCT/TTO的杀菌性能远高于SINC-TTO。Silica nanocapsules were synthesized in miniemulsion by using the surface of miniemulsion droplets as a template for the hydrolysis and condensation of tetraethoxysilane.In this study,we successfully used gemini emulsifier octenidine·2HCl(OCT)to encapsulate tea tree oil(TTO)in the core of silica nanocapsules(SINCOCT/TTO).Then,we synthesized the silica nanocapsule(SINC-TTO)in the same way,in which only OCT was replaced with another emulsifier cetyltrimethylammonium chloride(CTAC).We compared the SINC-OCT/TTO with SINC-TTO in the structure and properties.Fourier transform infrared(FT-IR),scanning electron microscopy(SEM),transmission electron microscopy(TEM)and thermogravimetric analysis(TGA)were used to characterize the structure of the nanocapsules.The specific surface areas and pore size of the SINCs were characterized with N2 absorptiondesorption.The hydrodynamic diameter of the capsules was evaluated by dynamic light scattering(DLS).The results showed that the content of TTO loaded in SINCs,hydrodynamic diameter,specific surface area and pore size of SINCs were controlled by the type of surfactant.The hydrodynamic diameter of the two kinds of nanocapsules were 67.36 nm and 121.7 nm,respectively,and the specific surface areas were 289 m^2/g and 437 m2/g,respectively.The mass fraction of TTO in SINC-OCT/TTO(47.7%)is higher than that in SINC-TTO(33.3%).The results of fractional inhibitory concentration index showed that TTO combined with OCT had synergistic antibacterial effects on S.aureus and had accumulative antibacterial effect on E.coli.The antibacterial experiment results of nanocapsules confirmed that the antibacterial effect of SINC-OCT/TTO was much higher than that of SINC-TTO.

关 键 词：茶树油 盐酸奥替尼啶 正硅酸乙酯 纳米胶囊 协同抗菌 

分 类 号：R751.05[医药卫生—皮肤病学与性病学]