声触诊组织成像定量技术诊断大鼠急性肝衰竭及评估预防性用药效果的价值  

Application value of virtual touch tissue imaging quantification technique in detecting acute liver failure and evaluating the effect of preventive medication in rats

作　　者：杜洋 土继政[1] 韩梅[1] 郝磊 郭西源 梁华 王兴华[1] DU Yang;TU Jizheng;HAN Mei;HAO Lei;GUO Xiyuan;LIANG Hua;WANG Xinghua(Department of Ultrasound,the Second Hospital of Shanxi Medical University,Taiyuan 030001,China)

机构地区：[1]山西医科大学第二医院超声科,太原市030001

出　　处：《临床超声医学杂志》2020年第10期721-724,共4页Journal of Clinical Ultrasound in Medicine

基　　金：山西省科技厅重点研发计划项目(201703D421029)。

摘　　要：目的探讨声触诊组织成像定量(VTIQ)技术在预测大鼠急性肝衰竭及评估预防性用药效果中的应用价值。方法将24只雄性SD大鼠随机分为对照组、模型未治疗组和模型治疗组,每组8只。模型治疗组予以枯草杆菌二联活菌肠溶胶囊(100 mg·kg^-1·d^-1)稀释后灌胃治疗,对照组和模型未治疗组每天给予同等量温生理盐水灌胃,共注射8 d;模型未治疗组和模型治疗组于实验第6、7天注射硫代乙酰胺(200 mg·kg^-1·d^-1)制备急性肝衰竭模型,共注射2次。应用VTIQ测量对照组和模型未治疗组、模型治疗组造模前后大鼠肝脏剪切波速度(SWV),比较上述参数的差异。以病理结果为金标准,绘制受试者工作特征(ROC)曲线,确定SWV预测急性肝衰竭的截断值并计算其曲线下面积。结果造模前对照组和模型未治疗组、模型治疗组大鼠肝脏平均SWV分别为(2.43±0.57)m/s、(2.61±0.42)m/s和(2.71±0.49)m/s,差异无统计学意义;造模后对照组、模型未治疗组和模型治疗组平均SWV分别为(2.50±0.71)m/s、(3.56±0.48)m/s和(3.06±0.27)m/s,模型未治疗组测值较对照组和模型治疗组升高,差异均有统计学意义(均P<0.05)。SWV预测大鼠急性肝衰竭的截断值为2.83 m/s,ROC曲线下面积、敏感性和特异性分别为0.938、100%和75.00%。结论VTIQ技术在预测大鼠急性肝衰竭及评估预防性用药效果方面具有一定的应用价值。Objective To investigate the clinical application value of virtual touch tissue imaging quantification(VTIQ)technique in detecting acute liver failure(ALF)and evaluating the effect of preventive medication in rats.Methods Twenty-four male SD rats were randomly divided into the control group,the model group and the treatment group with eight rats in each group.The treatment group was treated by gavage with Medilac-S(100 mg·kg^-1·d^-1)after dilution,while the control group and the model group were treated with the same volume of warm normal saline for 8 d.On the 6th and 7th day of the experiment,the model group and the treatment group were intraperitoneally injected with TAA(200 mg·kg^-1·d^-1).The shear wave velocity(SWV)of liver were measured and compared among three groups before and after the establishment of the model.Taken pathological result as the golden standard,ROC curve was drawn to obtain the cut-off value for predicting ALF by SWV,and area under the curve was calculated.Results Before modeling,the SWV mean of control group,model group and treatment group were(2.43±0.57)m/s,(2.61±0.42)m/s and(2.71±0.49)m/s,respectively,and the difference was not statistical significance.After modeling,the SWV mean of control group,model group and treatment group were(2.50±0.71)m/s,(3.56±0.48)m/s and(3.06±0.27)m/s,respectively.The SWV mean in the model group was higher than that in the control group,and the difference was statistical significance(P<0.05).With cut-off value of 2.83 m/s,the area under the curve,sensitivity and specificity were 0.938,100%and 75.00%in predicting ALF,respectively.Conclusion The VTIQ technique has a potential application value in predicting ALF and evaluating the effect of preventive medication.

关 键 词：超声检查 声触诊组织成像定量 肝衰竭 急性 大鼠 

分 类 号：R445.1[医药卫生—影像医学与核医学] R-332[医药卫生—诊断学]