HPLC测定金银花中绿原酸、新绿原酸和异绿原酸的含量  

Determination of Chlorogenic Acid,NeochloroGenic Acid and Isochlorogenic Acid in Honeysuckle by HPLC

作　　者：龚菊梅[1] 胡晓妹 何晓丽[1] 刘修树[1] 方丽波 范高福[1] 沈慧 范雪梅[3] GONG Jumei;HU Xiaomei;HE Xiaoli;LIU Xiushu;FANG Libo;FAN Gaofu;SHEN Hui;FAN Xuemei(Hefei Technology College,Hefei 230038,China;Putian City Hospital of Traditional Chinese Medicine,Putian 351100,China;Department of Chemistry,Tsinghua University,Beijing 100084,China)

机构地区：[1]合肥职业技术学院,安徽合肥230038 [2]福建省莆田市中医医院,福建莆田351100 [3]清华大学化学系,北京100084

出　　处：《安徽科技学院学报》2020年第4期53-57,共5页Journal of Anhui Science and Technology University

基　　金：安徽省自然科学重点项目(KJ2016A614,KJ2018A0825,KJ2019A1113)。

摘　　要：目的:建立同时测定金银花中绿原酸、新绿原酸和异绿原酸含量的方法。方法:采用高效液相色谱法。色谱柱为Phenomenex Luna C18柱(5μm,4.6 mm×250 mm),流动相为0.1%甲酸水溶液-乙腈(梯度洗脱),检测波长240 nm,柱温25℃。结果:绿原酸、新绿原酸和异绿原酸在0.013~0.526 mg/mL内呈良好线性关系(r为0.9993);平均加样回收率分别为99.53%、99.73%、100.23%,RSD分别为4.27%、2.49%、1.58%(n=6)。结论:该方法可靠、稳定、重现性好,可作为金银花中绿原酸、新绿原酸和异绿原酸的含量测定方法。Objective:To establish a method for simultaneous determination of chlorogenic acid,neochlorogenic acid and isochlorogenic acid in honeysuckle.Methods:HPLC method was adopted.The determination was performed on phenomenex luna C18(5μm,4.6 mm×250 mm)column with mobile phase consisted of 0.1%formic acid solution-acetonitrile(gradient elution).The detection wavelength was set at 250 nm and the column temperature was set at 25℃.Results:The linear ranges of chlorogenic acid,neochlorogenic acid and isochlorogenic acid were all 0.013-0.526 mg/mL(r=0.9993).The average recoveries of three compounds were 99.53%(RSD=4.27%,n=6),99.73%(RSD=2.49%,n=6),and 100.23%(RSD=1.58%,n=6),respectively.Conclusion:The method is feasible,stable,good reproducibility,for the determination of chlorogenic aeid,neochlorogenic acid and isochlorogenic acid in honeysuckle.

关 键 词：金银花 绿原酸 新绿原酸 异绿原酸 含量测定 

分 类 号：R917[医药卫生—药物分析学]