负载TGF-β3壳聚糖复合水凝胶促进兔半月板缺损修复  

Chitosan composite hydrogel loaded with TGF-β3 promotes the repair of meniscus defects in rabbits

作　　者：王赟 徐宏宇[3] 潘宗友 倪利承 王刚祥[3] 赵勇[3] 郑强[1] WANG Yun;XU Hong-yu;PAN Zong-you;NI Li-cheng;WANG Gang-xiang;ZHAO Yong;ZHENG Qiang(Department of Orthopaedics, the Second Affiliated Hospital, Medical College, Zhejiang University, Hangzhou 300009;Department of Orthopaedics, the First Affiliated Hospital, Medical College, Zhejiang University, Hangzhou 310003;Department of Orthopaedics, Shengzhou People's Hospital, Shengzhou 312400,China)

机构地区：[1]浙江大学医学院附属第二医院骨科,浙江杭州300009 [2]浙江大学医学院附属第一医院骨科,浙江杭州310003 [3]嵊州市人民医院骨科,浙江嵊州312400

出　　处：《基础医学与临床》2020年第11期1512-1518,共7页Basic and Clinical Medicine

摘　　要：目的研究骨髓间充质干细胞(BM-MSCs)与负载转化生长因子β3(TGF-β3)的壳聚糖复合水凝胶的复合物修复半月板缺损的效果。方法利用60只新西兰大白兔制作半月板前角全层缺损模型。将模型兔随机分为:对照组(未植入任何材料)、修复Ⅰ组(缺损处注入壳聚糖复合水凝胶与骨髓间充质干细胞复合物)、修复Ⅱ组(缺损处注入负载TGF-β3的壳聚糖复合水凝胶与骨髓间充质干细胞复合物)。术后12周取材,进行放射学检查、大体和组织学观察;利用Western blot检测Ⅱ型胶原、蛋白聚糖蛋白水平。结果对照组的半月板前角全层缺损,无明显修复迹象;修复Ⅰ组的缺损部位大部分被修复组织填充;修复Ⅱ组的缺损部位完全被修复组织填充。修复Ⅱ组的Ⅱ型胶原、蛋白聚糖蛋白表达高于对照组(P<0.05)。结论骨髓间充质干细胞与负载TGF-β3的壳聚糖水凝胶联合可促进兔半月板缺损的修复。Objective To study the effect of bone marrow mesenchymal stem cells(BM-MSCs)combined with chitosan composite hydrogel loaded with transforming growth factor beta 3(TGF-β3)to repair meniscus defects.Methods A full-thickness defect model of the anterior horn of the meniscus was developed in 60 New Zealand white rabbits.They were randomly divided into:the control group which did not implant any materials.The repair groupⅠin which chitosan composite hydrogel and bone marrow mesenchymal stem cells composite were injected into the defect group,and in groupⅡ,the chitosan composite hydrogel loaded with TGF-beta 3 and bone marrow mesenchymal stem cells complex were repaired.After 12 weeks of operation,the samples were taken for radiology,gross and histological observation.Western blot was used to detect typeⅡcollagen and proteoglycan protein.Results In the control group,the full-thickness defect of the meniscus anterior horn was not obviously repaired;in the repair groupⅠ,most of the defects were filled with repaired tissue;in the repair groupⅡ,the defects werecompletely filled with repaired tissue.The expression level of typeⅡcollagen and proteoglycan protein in repair groupⅡwas higher than that in control group(P<0.05).Conclusions Bone marrow mesenchymal stem cells combined with chitosan hydrogel loaded with TGF-beta 3 can promote the repair of meniscus defects in rabbits.

关 键 词：壳聚糖复合水凝胶 转化生长因子Β3 半月板缺损 Ⅱ型胶原 蛋白聚糖 

分 类 号：R683[医药卫生—骨科学]