山东烟区首次发现番茄斑萎病毒侵染  

The Infection of TSWV was First Found in Shandong Tobacco Growing Areas

作　　者：张万红 冯佳 ALI Kamran 罗健达 杨举田 王术科[3] 宗浩 申莉莉[1] 李莹 王凤龙[1] 张石飞 杨金广[1] 金轲 ZHANG Wanhong;FENG Jia;ALI Kamran;LUO Jianda;YANG Jutian;WANG Shuke;ZONG Hao;SHEN Lili;LI Ying;WANG Fenglong;ZHANG Shifei;YANG Jinguang;JIN Ke(Tobacco Research Institute of Chinese Academy of Agricultural Sciences,Qingdao 266101,China;Linyi Tobacco Company of Shandong Province,Linyi,Shandong 276001,China;Weifang Tobacco Company of Shandong Province,Weifang,Shandong 261061,China;Tobacco Company of Hongyunhonghe,Kunming 650231,China;Enshi Tobacco Leaf Branch Company,Enshi,Hubei 445000,China)

机构地区：[1]中国农业科学院烟草研究所,青岛266101 [2]山东临沂烟草有限公司,山东临沂276001 [3]山东潍坊烟草有限公司,山东潍坊261061 [4]红云红河烟草(集团)有限责任公司,昆明650231 [5]恩施市烟叶分公司,湖北恩施445000

出　　处：《中国烟草科学》2020年第5期87-91,共5页Chinese Tobacco Science

基　　金：中国烟草总公司烟草绿色防控重大专项“基于纳米传输系统的烟草病毒病免疫诱抗机理研究与应用”[110201901041(LS-04)]、“基于传播途径阻断的烟草斑萎病绿色防控关键技术研究与应用”[110202001033(LS-02)];四川省烟草公司科技项目“烟草病毒病快速检测技术的开发与应用”(SCYC201804);江西省烟草公司科技项目“江西病毒病绿色防控技术研究与应用”(2017.01.002)。

摘　　要：为确定番茄斑萎病毒(TSWV)在山东烟草上的侵染,基于GenBank中已有的TSWV基因组序列设计该病毒特异性引物,通过总RNA提取,RT-PCR扩增,全基因组序列测定和拼接分析等,结果表明,山东临沂疑似病样中含有TSWV,该TSWV分离物具有3条RNA链,大小分别为8911、4773和2971bp,与国内已报道的其他TSWV分离物核酸序列同源性均在99.0%以上,蛋白氨基酸序列同源性均在98.0%以上。系统进化分析结果显示,该分离物与已报道的TSWV云南分离物聚类到同一分支中,推测山东烟区中的TSWV可能由云南烟区通过带毒介体的迁入而传入。该研究为山东烟区TSWV的发生流行溯源和该危险性病毒病的精准测报及防控提供科学依据。In order to determine the infection of TSWV in Shandong tobacco,specific primers were designed based on the existing TSWV genome sequence in GenBank.Through total RNA extraction,RT-PCR amplification,whole genome sequence determination and splicing analysis,the results showed that the suspected samples of Linyi,Shandong province were infected by TSWV.In order to further determine the whole genome sequence of the TSWV Linyi isolates,TSWV whole genome amplification primers were designed and synthesized.The results of cloning,sequencing and splicing analysis showed that the three RNA chains segments of the Shandong isolate are 8911,4773 and 2971 bp in length,with more than 99.0%homology of nucleic acid sequence and 98.0%homology of protein sequence with the other reported Chinese isolates.Phylogenetic analysis showed that the Shandong isolate and the reported Yunnan TSWV isolate were clustered together in the same branch.It is speculated that TSWV in Shandong tobacco growing areas may have been introduced from Yunnan tobacco areas through the migration of toxic mediators.This study provides scientific evidence for the traceability of TSWV epidemics in Shandong tobacco growing areas and the accurate prediction and prevention of this dangerous viral disease.

关 键 词：番茄斑萎病毒(TSWV) 全基因组克隆 系统发育树 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]