生物玻璃/聚乳酸多孔微球的制备及其作为细胞载体的研究  

Bioglass/Polylactic Acid Porous Microspheres:Preparation and Their Application as Cell Microcarriers

作　　者：高龙 张赵文斌 常江[1,2] GAO Long;ZHANG Zhaowenbin;CHANG Jiang(Shanghai Institute of Ceramics,Chinese Academy of Sciences,Shanghai 200050,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院上海硅酸盐研究所,上海200050 [2]中国科学院大学,北京100049

出　　处：《无机材料学报》2020年第10期1163-1168,共6页Journal of Inorganic Materials

基　　金：国家重点研发计划(2016YFC1100201);中国科学院战略性先导科技专项(XDA16010203)。

摘　　要：具有大孔结构的多孔微球既可以在体外扩增细胞,还可以作为细胞的传输工具,通过注射的方式把细胞输送到需要修复的组织部位。生物玻璃虽然生物活性良好,但难以直接制备成大孔结构的微载体。因此,本研究将生物玻璃(BG)与聚乳酸(PLA)高分子复合,通过复乳法制备了一种含生物玻璃的多孔微球细胞微载体。并通过扫描电镜(SEM)、热重分析(TGA)、电感耦合等离子体发射光谱仪(ICP-OES)等方法研究分析了微球的形貌、组成和离子释放。通过细胞实验,证明细胞可以在微球的多孔结构中粘附和增殖,并且生物玻璃可以促进细胞增殖,在组织工程中具有潜在应用。Porous microsphere cell microcarrier with macroporous structure can not only amplify cells in vitro,but also serve as cell delivery tools to deliver cells to damaged tissues by injection.Bioglass(BG)is an inorganic material with excellent biological activity,however,it is difficult to directly prepare microcarriers with macroporous structure.Therefore,in this study,a BG/poly-lactic acid(PLA)porous microspheres was prepared by double emulsion method.The morphology and structure of the microsphere were characterized by SEM,and the load of BG in the microsphere was characterized by thermo gravimetric analysis and its ion release was detected by ICP.Cell proliferation experiments showed that cells could adhere and grow on the surface and inside of the microspheres.The results show that the microsphere with interconnected open-pore microstructure is suit for cell adhesion and proliferation.Bioglass can promote cell proliferation in the microspheres and the obtained BG/PLA composite microsphere has great potential applications in tissue engineering.

关 键 词：多孔微球 生物玻璃 聚乳酸 细胞微载体 组织工程 

分 类 号：R318.08[医药卫生—生物医学工程]