STAT3介导甲状旁腺激素调控张应力下牙槽骨塑建  

Intermittent parathyroid hormone promotes orthodontic tension force induced bone modeling via activating STAT3

作　　者：张程 吕春晓 李天成 陶贵渝 黄鹂[1] 邹淑娟[1] ZHANG Cheng;LV Chunxiao;LI Tiancheng;TAO Guiyu;HUANG Li;ZOU Shujuan(State Key Laboratory of Oral Diseases,Department of Orthodontics,West China Hospital of Stomatology,Sichuan University,610041 Chengdu,China)

机构地区：[1]口腔疾病研究国家重点实验室,四川大学华西口腔医院正畸科,成都610041

出　　处：《实用口腔医学杂志》2020年第5期736-739,共4页Journal of Practical Stomatology

基　　金：四川省科技厅科技计划项目(编号:2018JY0139)。

摘　　要：目的:探讨间歇性甲状旁腺激素(PTH)对正畸力作用下牙槽骨张力侧成骨活动的影响及其机制。方法:大鼠牙移动模型与成骨前体细胞系MC3T3-e1相结合,分为对照组、PTH组、PTH加Stattic(STAT3抑制剂)组。结果:免疫组化结果显示,14 d时PTH组大鼠牙移动张力侧牙槽骨表面及牙周膜成骨相关转录因子(OSX)及STAT3表达量显著增加,而Stattic抑制OSX和STAT3表达。PTH组MC3T3-e1中STAT3总蛋白和磷酸化蛋白(Tyr705)水平均上调,Col1a1、Osx、Alp、Runx2基因表达增加,PTH的作用可被Stattic削弱,差异有统计学意义。结论:间歇性PTH可能通过激活STAT3促进正畸张力侧牙槽骨的成骨活动。Objective:To investigate the influence of systemic intermittent parathyroid hormone(PTH)treatment on bone modeling at tension site during orthodontic tooth movement and its mechanics.Methods:In vivo experiment was conducted by establishing orthodontic tooth movement model in 24 wistar rats.In vitro study was carried out on mouse pre-osteoblastic cell line MC3T3-e1.The rats and cells were divided into blank control,PTH and PTH with Stattic(STAT3 inhibitor)groups.Results:In the in vivo study,immunohistochemistry showed that PTH promoted STAT3 and osterix(OSX)expression in osteoblasts and periodontal ligament at tension site of orthodontic tooth movement,which was weakened by local Stattic injection.In the in vitro test,PTH enhanced osteoblastic marker genes Col1a1,Osx,Alp,Runx2 expression level as well as total and phosphorylated STAT3(Tyr705)protein level,which were suppressed by Stattic treatment.Conclusion:Intermittent PTH treatment promotes orthodontic tension force induced bone modeling via activating STAT3.

关 键 词：牙移动 甲状旁腺激素肽(1-34) 骨生成 信号转导与转录激活因子3(STAT3) 

分 类 号：R783.5[医药卫生—口腔医学]