头穴丛刺对焦虑模型小鼠HPAA轴作用及相关分子机制研究  

Effect and Molecular Mechanism of Scalp Cluster Needling on HPAA Axis in Anxiety Mice

作　　者：王晨[1] 郑祖艳 苗永新[2] 张琳琳 WANG Chen;ZHENG Zuyan;MIAO Yongxin;ZHANG Linlin(Heilongjiang Provincial Hospital Affiliated to Harbin Institute of Technology, Harbin 150036, China;The First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin 150040, China)

机构地区：[1]哈尔滨工业大学附属黑龙江省医院,黑龙江哈尔滨150036 [2]黑龙江中医药大学附属第一医院,黑龙江哈尔滨150040

出　　处：《针灸临床杂志》2020年第9期70-73,共4页Journal of Clinical Acupuncture and Moxibustion

基　　金：哈尔滨市科技创新人才项目,编号:2016RAXYJ086。

摘　　要：目的:观察头穴丛刺对焦虑模型小鼠行为学及小鼠血浆和下丘脑中CRH、ACTH和CORT的改变,探论头穴丛刺对焦虑模型小鼠治疗作用的机制。SD小鼠随机分为针刺组、模型空白组和空白对照组。方法:采用高架迷宫实验(EPM)和间氯苯哌嗪(mCPP)共同诱导焦虑模型小鼠。分组后针刺组根据于氏头针丛刺法进行针刺,每日1次,每次15 min,连续治疗21 d;模型空白组与空白对照组同时进行与针刺组相同的固定,不进行针刺治疗。实验结束后复测焦虑模型小鼠行为学表现及通过免疫法和免疫组化法测下丘脑CRH、ACTH、CORT和NPY含量;RT-PCR法检测NPY受体Y1 mRNA、Y5 mRNA含量。结果:行为学测试:与模型空白组比较针刺组小鼠实验后进入开臂的时间与次数百分比(OT%及OE%)均明显升高,模型空白组与空白对照组对比时可发现,模型空白组小鼠的OT%、OE%值明显下降。与空白对照组比较,模型空白组小鼠血浆和下丘脑中CRH、ACTH、CORT浓度以及下丘脑NPY受体Y1 mRNA、Y5 mRNA浓度发生明显变化;与模型空白组比较,针刺组小鼠血浆和下丘脑中CRH、ACTH、CORT及NPY受体Y1 mRNA、Y5 mRNA的浓度明显改善。结论:头穴丛刺具有通过抑制焦虑模型小鼠HPAA轴兴奋性,降低CRF的分泌、改善焦虑症状的效果。Objective:To observe the behavioral changes and the levels of CRH,ACTH and CORT in plasma and hypothalamus,so as to discuss the therapeutic mechanism of scalp cluster needling on anxiety mice.Methods:SD mice were randomly divided into the acupuncture group,the model group and the control group.The model of anxiety mice was established by elevated plus-maze test(EPM)and injecting mCPP.The acupuncture group was treated with Yu’s scalp cluster needling,once a day,15min per time for 21 days,the mice in the other two groups were fixed without acupuncture at the same time point.The anxiety behavior was observed,the contents of CRH,ACTH,CORT and NPY in hypothalamus were detected by IHC method,and the expressions of Y1 mRNA and Y5 mRNA were detected by RT-PCR technique.Results:In terms of behavior results,compared to the control group,OT%and OE%were significantly decreased;after acupuncture intervention OT%and OE%were significantly increased.In terms of the contents CRH,ACTH,CORT and NPY,and the expressions of Y1 mRNA and Y5 mRNA,there were statistical differences between the model group and the control group;after acupuncture intervention,they were significantly improved.Conclusion:Scalp cluster needling has the function of decreasing CRF and relieving anxiety by restrain the HPAA axis in anxiety mice.

关 键 词：焦虑 小鼠 头穴 丛刺 HPAA轴 高架迷宫实验 促肾上腺皮质激素释放因子 

分 类 号：R246.6[医药卫生—针灸推拿学]