新鲜血小板、冰冻血小板及两者混合后的TEG参数分析及其临床意义  

Analysis of TEG parameters of fresh platelets,frozen platelets and their mixed and its clinical significance

作　　者：齐峰 刘欢[1] QI Feng;LIU Huan(Department of Blood Transfusion,Affiliated Hospital of Yan an University,Yan’an 716000,Shaanxi,China)

机构地区：[1]延安大学附属医院输血科,陕西延安716000

出　　处：《川北医学院学报》2020年第5期895-897,共3页Journal of North Sichuan Medical College

摘　　要：目的:探究新鲜血小板、冰冻血小板及两者混合后的血栓弹力图(TEG)参数状况及其临床意义。方法:选择5%二甲基亚砜(DMSO)制备保存于-80℃的冰冻血小板及72 h内采集的新鲜血小板作为本次研究对象。将其中40例新鲜血小板作为A组;新鲜血小板与冰冻血小板按2∶1的比例混合制备40例作为B组;新鲜血小板与冰冻血小板按1∶1的比例混合制备40例作为C组;40例冰冻血小板作为D组。分别对4组标本进行TEG参数检测,分析反应时间、凝血时间、α角、最大振幅各项参数水平,并对4组标本常规参数进行检测,分析血小板功能情况。结果:4组标本TEG参数检测显示,反应时间:D组>B组>C组>A组;凝血时间:C组>B组>D组>A组;α角:B组>C组>A组>D组;最大振幅:B组>C组>D组>A组,差异均有统计学意义(P<0.05);4组标本血小板常规参数检测显示,血小板计数:A组>B组>C组>D组,平均血小板体积:D组>C组>B组>A组,血小板分布宽度:D组>C组>B组>A组,差异具有统计学意义(P<0.05)。结论:新鲜血小板与冰冻血小板按照1∶1的比例进行混合后输注有助于缩短血液凝固时间,对患者血块凝集的影响较小,是一种理想的输血模式,值得在临床治疗中推广。Objective:To explore the analysis of thromboelasticity(TEG)parameters of fresh platelets,frozen platelets,and the mixture and their clinical significance.Methods:The frozen platelets stored at-80℃and fresh platelets collected within 72 hours were selected from 5%dimethyl sulfoxide(DMSO).40 cases of fresh platelets were taken as group A.Fresh platelets and frozen platelets were mixed at a ratio of 2∶1 to prepare 40 cases as group B.Fresh platelets and frozen platelets were mixed at a ratio of 1∶1 to prepare 40 cases as group C and 40 cases Frozen platelets were used as group D,and TEG parameters were measured on 4 groups of samples,and the response time(R),coagulation time(K),αangle(Ang),and maximum amplitude(MA)were analyzed.The routine parameters were tested to analyze platelet function.Results:The TEG parameters of the four groups of specimens showed that the response time:group D>group B>group C>group A,coagulation time:group C>group B>group D>group A,αangle:group B>group C>group A>Group D,the maximum amplitude:group B>group C>group D>group A,the differences were statistically significant(P<0.05),The routine platelet parameters of four groups of specimens showed that platelet count group A>group B>group C>group D,mean platelet volume:group D>group C>group B>group A,platelet distribution width:group D>group C>group B>group A,the differences were statistically significant(P<0.05).Conclusion:The mixed transfusion of fresh platelets and frozen platelets at a ratio of 1∶1 helps to shorten blood clotting time and has less effect on the patient s blood clot,and it is an ideal blood transfusion mode and deserves widespread promotion in clinical treatment.

关 键 词：新鲜血小板 冰冻血小板 血栓弹力图 临床价值 

分 类 号：R457.1[医药卫生—治疗学]