Green coffee bean hydroalcoholic extract accelerates wound healing in full-thickness wounds in rabbits  

作　　者：Reza Shahriari Pari Tamri Amir Larki Harchegani Alireza Nourian 

机构地区：[1]Department of Pharmacology&Toxicology,School of Pharmacy,Medicinal Plants and Natural Products Research Center,Hamadan University of Medical Sciences,Hamadan 6517838678,Iran [2]Department of Laboratory Sciences,Faculty of Para-Veterinary Sciences,Bu-Ali Sina University,Hamadan 6517838695,Iran

出　　处：《Traditional Medicine Research》2020年第6期433-441,共9页TMR传统医学研究

基　　金：This study was financially supported by Vice-Chancellor of Research and Technology,Hamadan University of Medical Sciences,Hamadan,Iran(Gran no,9509095210).

摘　　要：Background:Green coffee beans contain biochemical compounds including caffeine,chlorogenic acid,trigonelline,and diterpenoid alcohols.In traditional Iranian medicine,coffee bean powder is used for the treatment of wounds.Previous studies have shown that green coffee bean extract has a number of health benefits,including induction of weight loss,reduced blood pressure,and hepatoprotective and anti-Parkinson effects.The aim of this study was to investigate the wound-healing activity of green coffee bean extract on a full-thickness wound model.Methods:Full-thickness wounds of 20×20 mm were created on the back of New Zealand white rabbits.The animals were divided into 6 groups.Three concentrations of green coffee bean extract(5%,10%,and 15%w/w)in a eucerin base were applied over the wounds in 3 test groups.One percent phenytoin cream was used in one group as a positive control.The rabbits of negative control and vehicle groups received no treatment and eucerin,respectively.For the evaluation of green coffee bean extract’s wound-healing effects,measures included:(1)wound-closure rate,by daily measuring of the wound surface area and calculating the reduction in area;(2)period of epithelialization,the number of days until the scab dropped from the wound;(3)hydroxyproline content,measured by a hydroxyproline assay kit;(4)lipid peroxidation,measured by a lipid peroxidation assay kit;and(5)histopathological state(hematoxylin-eosin stain)of wound-tissue samples on days 7 and 14 post-wounding.Results:The results of this study showed significantly enhanced wound-closure rate,shorter period of epithelialization(P<0.01),increased hydroxyproline content(P<0.001)and suppressed lipid peroxidation(P<0.001 on day 14)of wounds on animals treated with the 10%green coffee bean extract compared to the negative control and eucerin-treated groups.Moreover,the therapeutic effects of 10%green coffee bean were significantly superior than those of phenytoin on enhancing wound-closure rate,decreasing period of epithelialization(P<0.05),incr

关 键 词：Green coffee bean Green coffee bean extract Wound healing EPITHELIALIZATION HYDROXYPROLINE Histopathology 

分 类 号：O62[理学—有机化学]