基于线粒体12S rRNA基因的大鹏半岛海域石珊瑚的系统发育分析  

12S rRNA-based Phylogenetic Analysis of Scleractinia from Dapeng Peninsula

作　　者：肖宝华 廖宝林 王琼 谢子强 谢勇琪 覃业曼 XIAO Baohua;LIAO Baolin;WANG Qiong;XIE Ziqiang;XIE Yongqi;QIN Yeman(Shenzhen Institute of Guangdong Ocean University,Guangdong Ocean University,Shenzhen Guangdong 518000,China;Shenzhen Ocean Hyaline Marine Science and Technology Co.Ltd,Shenzhen Guangdong 518000,China)

机构地区：[1]广东海洋大学深圳研究院,广东深圳518000 [2]深圳市碧海蓝天海洋科技有限公司,广东深圳518000

出　　处：《海南热带海洋学院学报》2020年第5期29-35,共7页Journal of Hainan Tropical Ocean University

基　　金：广东省渔港建设和渔业产业发展专项(A201708D06);广东省促进经济高质量发展专项资金项目〔GDOE(2019)A01〕;深圳市科技研发资金项目(KJYY20180213182720347)。

摘　　要：通过12S rRNA基因特异性扩增测序,对采自深圳大鹏半岛海域的16个石珊瑚样品的12S rRNA基因进行系统进化分析.研究结果表明:供试的16个样品来源于9种石珊瑚,12S rRNA序列的平均G+C含量为37.7%.通过最大似然法(ML)、邻位连接法(NJ)和最小进化法(ME)构建的系统发育树将9种石珊瑚聚类为2个大的分支:滨珊瑚科、菌珊瑚科、枇杷珊瑚科和鹿角珊瑚科的7种石珊瑚聚类一个分支;蜂巢珊瑚科的2种石珊瑚聚类为另一个分支,处于进化树基部.这2个分支的聚类验证了之前关于石珊瑚坚实型和复合型类群分类的研究.对比其他石珊瑚分子标记,本研究中的12S rRNA基因序列长度较长、基因变化速率快,分化程度较高,能够作为科以下亲缘关系鉴定的有效分子标记.Sequences of 12S rRNA from 16 scleractinian samples in the water of Dapeng peninsula were amplified and sequenced.Moreover,the base ratio and genetic distances were analyzed.The result indicated that 16 scleractinian samples were identified as 9 species and the average G+C content of 12S rRNA is 37.7%.The 12S rRNA data was used to construct phylogeny trees of scleractinian.According to the Neighbor-joining,Maximum-likelihood and Minimum-evolution trees,9 species of scleractinian could be divided into two clades.The separated Faviidae was the root clade,and the other clade was composed of Poritidae,Agariciidae,Acroporidae and Oculinidae.The result confirmed previous study about robust and complex group of scleractinian.The molecular marker of scleractinian 12S rRNA used in this study has faster genomic evolutionary rate and better distinguishable susceptibility.

关 键 词：石珊瑚 12S rRNA 分子系统学 

分 类 号：Q75[生物学—分子生物学] Q178.53