基于HPLC特征图谱、多成分定量结合化学计量学方法评价不同采收期甘草药材的质量  

Quality Evaluation of Glycyrrhiza uralensis Fisch.in Different Harvest Periods Based on Combinative Methods of HPLC Specific Chromatogram,Multi-Component Assay,and Chemometrics

作　　者：陈佳[1] 张权 赵莎 尚兴朴 杜杰 王继永 魏锋[1] 马双成[1] CHEN Jia;ZHANG Quan;ZHAO Sha;SHANG Xing-pu;DU Jie;WANG Ji-yong;WEI Feng;MA Shuang-cheng(National Institutes for Food and Drug Control,Beijing 100050,China;China National Traditional Chinese Medicine Co.,Ltd.,Beijing 102600,China)

机构地区：[1]中国食品药品检定研究院,北京100050 [2]中国中药有限公司科技研发部,北京102600

出　　处：《中国药学杂志》2020年第18期1540-1547,共8页Chinese Pharmaceutical Journal

基　　金：国家十二五“重大新药创制”专项资助(2014ZX09304307-002);国家中药标准化项目资助(ZYBZH-Y-ZY-45)。

摘　　要：目的基于HPLC特征图谱及多成分定量,采用化学计量学分析方法综合评价不同采收期甘草药材的质量。方法采用中药色谱指纹图谱相似度评价系统(2012版)对不同采收期甘草药材的HPLC特征图谱进行相似度评价;运用t检验、相关性分析、聚类分析(clustering analysis,HCA)、主成分分析(principal component analysis,PCA)和偏最小二乘法-判别分析(partial least-squares discriminant analysis,PLS-DA)对数据进行统计。结果选取21个色谱峰作为指纹图谱共有峰,样品相似度均在0.9以上;测定了甘草苷、甘草酸、芹糖甘草苷、芹糖异甘草苷、异甘草苷、甘草素、新异甘草苷和刺甘草查耳酮8种化学成分的含量。化学计量学分析表明,不同采收期甘草药材质量存在一定差异,芹糖甘草苷、新异甘草苷和刺甘草查耳酮是甘草药材不同采收期的差异化合物。结论秋季是甘草药材最佳采收期。建议将芹糖甘草苷作为评价不同采收期甘草药材的关键性质量控制指标。本实验为不同采收期甘草药材的质量控制提供了数据支撑和科学依据。OBJECTIVE To evaluate comprehensively the quality of Glycyrrhiza uralensis Fisch.in different harvest periods by chemometric analysis based on the HPLC specific chromatogram and multi-component assay.METHODS The similarity was analyzed with“Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica 2012”.t-test,correlation analysis,clustering analysis(HCA),principal component analysis(PCA)and partial least-squares discriminant analysis(PLS-DA)were used for data analysis.RESULTS Twenty-one peaks were selected as common peaks of the fingerprint.The similarity of the samples were above 0.9.The contents of liquiritin,glycyrrhizic acid,liquiritin apioside,isoliquiritin apioside,isoliquiritin,neoisoliquiritin,echinatin,and liquiritigenin were determined.There were some differences in the quality of Glycyrrhiza uralensis Fisch.in different harvest periods,with liquiritin apioside,neoisoliquiritin and echinatin as the main compounds of difference.CONCLUSION Autumn is confirmed as the best harvesting period of Glycyrrhiza uralensis Fisch.by chemometric analysis.It is suggested that liquiritin apioside should be used as the key quality control indicator for evaluating Glycyrrhiza uralensis Fisch.in different harvest periods.

关 键 词：甘草 采收期 特征图谱 多成分定量 化学计量学 质量评价 

分 类 号：R284[医药卫生—中药学]