肝郁证雌性大鼠的神经内分泌状态及柴胡疏肝散的干预作用  

Changes of Neuroendocrine Function in Female Rats with Gan Depression Syndrome and Intervention of Chaihu Shugan Powder

作　　者：张媛凤 钱梦 杨宗纯 谢鸣 ZHANG Yuan-feng;QIAN Meng;YANG Zong-chun;XIE Ming(Department of Formulary,School of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Bei-jing,100029)

机构地区：[1]北京中医药大学中医学院方剂学系,北京100029

出　　处：《中国中西医结合杂志》2020年第10期1246-1251,共6页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.81173193)。

摘　　要：目的探讨肝郁证雌性大鼠的神经内分泌状态及因证治方柴胡疏肝散的干预作用。方法Wistar雌性大鼠随机分为正常对照,模型组,柴胡疏肝散大、小剂量组共4组,每组10只。慢性束缚法复制肝郁证模型,连续4周。2个中药干预组大鼠在造模第2周末,分别按1.26 g/(kg·d)和0.31 g/(kg·d)剂量给予柴胡疏肝散灌胃,每日1次,连续2周;模型组和正常对照组大鼠给予等量蒸馏水。采用放射免疫法测定下丘脑组织促肾上腺素皮质激素释放激素(CRH)、促甲状腺释放激素(TRH)、促性腺激素释放激素(GnRH)。采用酶联免疫法测定血浆去甲肾上腺素(NE)、肾上腺素(E)、五羟色胺(5-TH)、多巴胺(DA)、β内啡肽(β-EP);采用放射免疫法测定血清促肾上腺皮质激素(ACTH)、皮质酮(CORT),促甲状腺素(TSH)、三碘甲状腺原氨酸(T3)、甲状腺素(T4),卵泡刺激素(FSH)、黄体生成素(LH)、泌乳素(PRL)、孕酮(P)、雌二醇(E_2)、睾酮(T)。结果与正常组比较,模型组大鼠血浆5-HT、DA、NE降低,血浆E、β-EP升高(P<0.05,P<0.01);下丘脑组织CRH、TRH,血清ACTH、CORT、T3、T4均升高,血清TSH降低(P<0.01);下丘脑组织GnRH,血清FSH、LH、PRL、P、T、E_2均升高(P<0.05,P<0.01)。与模型组比较,柴胡疏肝散大剂量组血浆5-HT、NE升高,E、β-EP降低(P<0.05,P<0.01),下丘脑组织CRH,血清ACTH、CORT降低(P<0.01),下丘脑组织TRH,血清T3、T4降低,TSH升高(P<0.01),下丘脑组织GnRH,血清FSH、LH、PRL、P、T均降低(P<0.05,P<0.01);柴胡疏肝散小剂量组大鼠下丘脑CRH,血浆β-EP和血清ACTH、T4、FSH、LH、PRL、P、E_2均降低(P<0.05,P<0.01),血浆DA升高(P<0.01)。结论慢性束缚法雌性肝郁模型大鼠存在神经内分泌功能的失调,表现为下丘脑-垂体-肾上腺皮质/甲状腺/性腺轴的功能亢进,并伴有下丘脑和(或)垂体的负反馈调节障碍。柴胡疏肝散对该模型大鼠神经-内分泌轴的异常具有一定的调节作用,其大剂量作用优于�Objective To observe the changes of neuroendocrine function in female rats with Gan depression syndrome(GDS),and intervention of Chaihu Shugan Powder(CHSGP).Methods Female Wistar rats were randomly divided into the normal control group(C),GDS model group(M),high dose CHSGP group(H),and low dose CHSGP group(L),10 in each group.Chronic restraint stress was used to duplicate GDS model for 4 successive weeks.At the end of the 2 nd week of modeling,rats in the H group and L group were administered with CHSGP at the daily dose of 1.26 g/kg and 0.31 g/kg by gastrogavage,once per day for 2 successive weeks.The indicators such as corticotropin releasing hormone(CRH),thyrotrophic-releasing hormone(TRH),and gonadotropin-releasing hormone(GnRH)were determined using radioimmunoassay.Contents of plasma noradrenalin(NE),epinephrine(E),5-hydroxytryptamine(5-HT),dopamine(DA),andβ-endorphin(β-EP)were determined using ELISA.Contents of serum adrenocorticotropic hormone(ACTH),corticosterone(CORT),thyroid stimulating hormone(TSH),triiodothyronine(T3),thyroxine(T4),follicle-stimulating hormone(FSH),luteotropic hormone(LH),prolactin(PRL),progesterone(P),estradiol(E2),and testosterone(T)were determined using radioimmunoassay.Results Compared with the C group,plasma contents of 5-HT,DA,and NE decreased,and plasma contents of E andβ-EP increased in the M group(P<0.05,P<0.01).CRH and TRH contents in hypothalamus,serum levels of ACTH,CORT,T3,and T4 all increased,serum TSH level decreased in the M group(P<0.01).GnRH content in hypothalamus,serum levels of FSH,LH,PRL,P,T,and E2 all increased(P<0.05,P<0.01).Compared with the M group,plasma contents of 5-HT and NE increased,plasma contents of E andβ-EP decreased(P<0.05,P<0.01);CRH content in hypothalamus,serum levels of ACTH and CORT decreased(P<0.01);TRH content in hypothalamus,serum levels of T3 and T4 decreased,and TSH increased(P<0.01);GnRH content in hypothalamus,serum levels of FSH,LH,PRL,P,and T all decreased in the H group(P<0.05,P<0.01).CRH content in hypothalamus,plasmaβ-EP conte

关 键 词：雌鼠 肝郁证 下丘脑-垂体-肾上腺/甲状腺/性腺轴 柴胡疏肝散 

分 类 号：R285.5[医药卫生—中药学]