表面活性剂协同超声波酶法提取三七花总皂苷工艺优化及抗氧化活性研究  

Enzymolysis-ultrasound-assisted surfactant extraction and antioxidant activity of saponins from Panax notoginseng flowers

作　　者：陈红惠 TARUN BELWAL 李刚凤 罗自生[2,3,4] CHEN Honghui;TARUN BELWAL;LI Gangfeng;LUO Zisheng(College of Chemistry and Engineering,Wenshan University,Wenshan 663099,China;College of Biosystems Engineering and Food Science,Zhejiang University,Hangzhou 310058,China;Fuli Institute of Food Science,Zhejiang University,Hangzhou 310058,China;Key Laboratory of Agro-Products Postharvest Handling,Ministry of Agriculture and Rural Affairs,China National-Local Joint Engineering Laboratory of Intelligent Food Technology and Equipment,Zhejiang Key Laboratory for Agro-Food Processing,Zhejiang Engineering Laboratory of Food Technology and Equipment,Zhejiang University,Hangzhou 310058,China)

机构地区：[1]文山学院化学与工程学院,云南文山663099 [2]浙江大学生物系统工程与食品科学学院,浙江杭州310058 [3]浙江大学馥莉食品研究院,浙江杭州310058 [4]浙江大学农业和农村部农产品采后处理重点实验室、国家和地方食品智能技术与装备联合工程实验室、浙江省农产品加工重点实验室、浙江省食品技术与装备工程实验室,浙江杭州310058

出　　处：《食品与发酵工业》2020年第20期178-186,共9页Food and Fermentation Industries

基　　金：浙江省重点研发项目(2020C02040);云南省教育厅科学研究项目(2019J0908)。

摘　　要：建立并优化了表面活性剂协同超声波酶法提取三七花总皂苷的工艺条件。以Triton X-100表面活性剂为提取溶剂,在复合酶添加量为0.2%(质量分数),液固比为30∶1(mL∶g),Triton X-100溶液pH值为5时,表面活性剂协同超声波酶法提取三七花总皂苷最优条件为:酶解温度60℃,超声功率465 W,超声40 min,此时三七花总皂苷的提取率为16.38%,高于传统的回流加热提取、超声波辅助提取。对3种工艺提取三七花总皂苷进行抗氧化活性测定,结果表明,表面活性剂协同超声波酶法提取得到的三七总皂苷对1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基清除能力更高,总抗氧化能力更强,其质量浓度为0.156 mg/mL时,对DNA损伤保护作用最强。该研究方法萃取效率较高,环保安全,易于推广应用。An enzymolysis-ultrasound-assisted surfactant extraction method was developed and optimized to enhance the saponins yield from Panax notoginseng flowers.The Triton X-100 surfactant was used as extraction solvent.When the enzyme concentration was 0.2%,solvent to sample ratio at 30∶1(mL∶g)and Triton X-100 solution at pH 5,the optimal extraction condition of enzymolysis-ultrasound-assisted surfactant extraction was as follows:Enzymolysis temperature at 60℃and ultrasonication power was 465 W for 40 min.Under the optimized condition,the yield of total saponin was 16.38%,which is significantly higher than that of traditional heat reflux extraction and ultrasound-assisted extraction methods.In addition,the antioxidant activity of Panax notoginseng saponins extracted by three processes showed that the Panax notoginseng saponins extracted by enzymolysis-ultrasound-assisted surfactant extraction method had higher scavenging ability of DPPH free radicals and stronger total antioxidant capacity.In case of protecting activity against DNA damage,0.156 mg/mL of saponins was optimum.Our finding clearly demonstrated that the developed novel enzymolysis-ultrasound-assisted surfactant extraction method could be applied to gain higher extraction efficiency of saponins,and the developed method is environmentally safe and easy to be scaled up.

关 键 词：表面活性剂 超声波 酶解 三七花 总皂苷 

分 类 号：R284.2[医药卫生—中药学] R285[医药卫生—中医学]